Strain Name: Sanger MirKO ES cell line Mir23b/Mir27b cluster 13N2
Stock Number: 036364-UCD
Other Names:
- 13N2
Gene Details: (provided by MGI)
| **This cell line is a cluster knockout in which more than one gene has been targeted** |
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Gene Symbol: Mir23b Name: microRNA 23b Alteration at locus: Knockout |
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Gene Symbol: Mir27b Name: microRNA 27b Alteration at locus: Knockout |
Genetic Alterations:
The cluster of microRNA genes was replaced via homologous recombination by a
LoxP-F3-PGK-EM7-PuroΔtk-bpA-LoxP-FRT targeting vector with
5.5kb (5’) and 3.3kb (3’) arms of homology. Briefly, this is a
puromycin/thymidine kinase fusion gene under the control of EM7/PGK
promoters and flanked by LoxP and F3/FRT sites. Schematic of MirKO targeting vector and alleles
Mouse strain from which the parental cell line was derived:
Related MMRRC Products:
Mir23b: MMRRC:036363-UCD Sanger MirKO ES cell line Mir23b-Mir24-1 cluster 13N5
Mir23b: MMRRC:036865-UCD Keck MirKO ES cell line Mirc22
Please see also: Sanger MicroRNA Knockout ES Cell Lines Overview
Genotype Determination:
Related Protocols:
- Cell Culture related publication: Pettitt et al, 2009
- IKMC MartSearch - information available such as vector design, PCR results, and gene details
- Mirbase.org - searchable database of published miRNA sequences and annotation
- EBI MicroCosm Targets - contains computationally predicted targets for microRNAs
Strain Origin
Donor: Allan Bradley, PhD, Wellcome Trust Sanger Institute, UK
Primary Reference:
Prosser HM, Koike-Yusa H, Cooper JD, Law FC, Bradley A. A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nature Biotechnology 2011, 29(9):840-5. (Medline PMID:21822254).
Special Considerations
Health Status Report
Requested cell lines are tested by PCR analysis for mycoplasma spp. and Murine Parvoviruses (MPV) upon completion of expansion and prior to shipment. Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email mmrrc@ucdavis.edu.
Order Request Information
Availability Level:
Cryo-preserved ES cell line material can usually be shipped in 2 to 4 weeks. Genetic confirmation adds an additional 4 to 6 weeks (but is recommended to confirm that the material has not been contaminated or otherwise incorrectly identified). The microinjection services add an additional 10 to 12 weeks and can vary from cell line to cell line.
Conditions of Distribution:
Please complete the MMRRC Conditions of Use (COU) and provide it at the time your order is placed. Note: The preceding link is to the COU text only; the COU user form will be provided after order submission.
Fees:
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
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- Products for this strain are Not Yet Available for
Ordering - If you register interest in this strain, you will be notified when it becomes available for ordering. | |||||||
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Distribution Fee/unit (US $) |
Units | Notes | ||||
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| Vial | Without sequence confirmation.* | ||||
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| per cell line | Genetic confirmation recommended.*Service fee is in addition to fee for mutant cell line. | ||||
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| per cell line | In addition to the per mutant ES cell line fee (see above); chimeras not guaranteed. | ||||
*Genetic confirmation of a cell line is an add-on service; cost is in addition to the cost of the cell line itself. We recommend minimum verification of the cell line by genetic confirmation of the reporter sequence. Please inquire with the supplying MMRRC facility when ordering (mmrrc@ucdavis.edu).
This strain is not currently available. If you register interest in this strain, you will be notified when it becomes available for ordering (click Register Interest button above).
The MMRRC is a collaborative effort, funded by grants from the NIH
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