Strain Name: SIGTR ES cell line XP0209
Stock Number: 025459-UCD
Other Names:
- XP0209
Gene Details: (provided by MGI)
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Gene Symbol: Sumo3 Name: SMT3 suppressor of mif two 3 homolog 3 (yeast) Alteration at locus: Gene Trap |
| Chromosome: 10 |
Genetic Alterations:
High throughput gene trapping was performed by inserting a gene trap vector
containing a splice-acceptor sequence upstream of a reporter gene, β-geo
(a fusion of β-galactosidase and neomycin phosphotransferase II), into
an intronic or coding region of genomic DNA. The resulting insertional
mutation creates a fusion transcript containing sequences from exons
upstream of the insertion joined to the β-geo marker, allowing cell
lines where the vector has successfully interrupted a gene to be
identified.
Mouse strain from which the parental cell line was derived:
Related MMRRC Products:
Sumo3: MMRRC:006713-UCD BayGenomics ES cell line XB039
Sumo3: MMRRC:006758-UCD BayGenomics ES cell line XB216
Sumo3: MMRRC:017883-UCD SIGTR ES cell line AW0265
Sumo3
Please also see: MMRRC Overview of the Sanger Institute Gene Trap Resource ES Cell Line collection
Check to access IGTC Cell Line Annotation
Related Protocols:
- MMRRC's Cell Culture Protocol
- See also Sanger's Scientific Resources page, especially the "Handling cell lines" tab.
Strain Origin
Donor: Wellcome Trust Sanger Institute, The Sanger Institute Gene Trap Resource (SIGTR), Cambridge, UK
Primary Reference:
Skarnes WC, von Melchner H, Wurst W, Hicks G, Nord AS, Cox T, Young SG, Ruiz P, Soriano P, Tessier-Lavigne M, Conklin BR, Stanford WL, Rossant J, International Gene Trap Consortium. A public gene trap resource for mouse functional genomics. Nat Genet. 2004 Jun;36(6):543-4. (Medline PMID: 15167922)
Special Considerations
When choosing a clone, we recommend blasting the sequence tag to estimate the location of a gene trap. Generally you will want to select a clone that traps the furthest upstream. The vast majority of gene trap cell lines have been correctly identified, as judged by DNA sequencing (i.e., repeat determination of the DNA sequence tag when the ES cell clone is requested). However, incorrectly identified ES cell clones are occasionally encountered, either because of human error or because a thawed ES cell line gets overgrown by a contaminating ES cell line. Therefore, we strongly recommend that you request verification of the identity of your ES cell line by DNA sequencing (there is an additional fee for DNA sequence verification of your clone). We also recommend that recipients of ES cells re-verify the identity of the ES cell line by RT-PCR and/or Southern blot analysis. If more than one ES cell line is available for your "gene-of-interest", we recommend that you order several of the ES cell lines. Ordering more than one ES cell line for a specific "gene-of-interest" will increase the chances of success.
Health Status Report
Requested cell lines are tested by PCR analysis for mycoplasma spp. and Murine Parvoviruses (MPV) upon completion of expansion and prior to shipment. The parental cell line E14Tg2a.4, from which these cells were derived, was tested for multiple mouse pathogens; please see the Health Status Report. Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email mmrrc@ucdavis.edu.
Order Request Information
Availability Level:
Cryo-preserved ES cell line material can usually be shipped in 2 to 4 weeks. Genetic confirmation adds an additional 4 to 6 weeks (but is recommended to confirm that the material has not been contaminated or otherwise incorrectly identified). The microinjection services add an additional 10 to 12 weeks and can vary from cell line to cell line.
Conditions of Distribution:
Please complete the Sanger Institute Gene Trap MTA. This document must be signed by an authorized Technology Transfer representative from your institution. Please provide two signed originals, mailed to the address located on page 2 of the MTA. The MTA will be counter-signed by a designated representative from the MMRRC, and a fully executed copy returned to your institution. Your request will be processed upon receipt of the signed MTA at the appropriate MMRRC center.
Fees:
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
| Click button to Request this one strain.
(Use the MMRRC Catalog Search to request more than one strain.) | |||||||
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Distribution Fee/unit (US $) |
Units | Notes | ||||
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| Vial | Without sequence confirmation.* | ||||
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| per cell line | Genetic confirmation recommended.*Service fee is in addition to fee for mutant cell line. | ||||
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| per cell line | In addition to the per mutant ES cell line fee (see above); chimeras not guaranteed. | ||||
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| per cell line | In addition to the per mutant ES cell line fee (see above); at least one 50% or greater chimeric male, or not charged at all. | ||||
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| Inquire at mmrrc@ucdavis.edu | |||||
*Genetic confirmation of a cell line is an add-on service; cost is in addition to the cost of the cell line itself. We recommend minimum verification of the cell line by genetic confirmation of the reporter sequence.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-7510 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.
The MMRRC is a collaborative effort, funded by grants from the NIH
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