Strain Detail Sheet

Strain Name: Nagy ES cell line G4

Stock Number: 011986-MU

Other Names:

Gene Details: (provided by MGI)

Alteration at locus: Non-mutant

Genetic Alterations:

Genotype Determination:

    Parental ES Cell Line: G4
    Mouse strain from which the parental cell line was derived: 129S6/SvEvTac X C57BL/6Ncr F1


    Related Protocols:

    Strain Origin

    Donor: Andras Nagy, Ph.D., Mt. Sinai Hospital, Toronto

    Primary Reference:

    • Vintersten K, Monetti C, Gertsenstein M, Zhang P, Laszlo L, Biechele S, Nagy A. Mouse in red: red fluorescent protein expression in mouse ES cells, embryos, and adult animals. Genesis. 2004 Dec;40(4):241-6. Erratum in: Genesis. 2005 Jul;42(3):218. (Medline PMID: 15593332)
    • George SH, Gertsenstein M, Vintersten K, Korets-Smith E, Murphy J, Stevens ME, Haigh JJ, Nagy A. Developmental and adult phenotyping directly from mutant embryonic stem cells. Proc Natl Acad Sci U S A. 2007 Mar 13;104(11):4455-60. Epub 2007 Mar 2. (Medline PMID: 17360545)

    Special Considerations

    This embryonic cell line is suitable for use in homologous recombination protocols.

    Supplemental Information

    Related ES cell lines:
    MMRRC:011987, Nagy ES cell line G4 with Z/red transgene
    MMRRC:011988, Nagy ES cell line G4 with DsRed.MST transgene

    Health Status Report

    Requested cell lines are tested by PCR analysis for mycoplasma spp. and Murine Parvoviruses (MPV) upon completion of expansion and prior to shipment. Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email mmrrc@missouri.edu.

    Order Request Information

    Availability Level:

    Cryo-preserved ES cell line material can usually be shipped in 2 to 4 weeks.

    Conditions of Distribution:

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Fees:

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description
    		Distribution
    Fee/unit (US $)    		 Units Notes
    011986-MU-CELLNagy Basic ES cell line G4
    $546.00
    Non-Profit
    		 Vial Without sequence confirmation.*

    *

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-7510 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.

    The MMRRC is a collaborative effort, funded by grants from the NIH

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