Strain Name: Nagy ES cell line G4 with Z/red transgene
Stock Number: 011987-MU
Other Names:
Gene Details: (provided by MGI)
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Enhancer: CMV (Viral) Name: cytomegalovirus Alteration at locus: Transgenic |
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Promoter: ACTB (Chicken) Name: actin, beta Alteration at locus: Transgenic |
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Reporter: lacZ (E. Coli) Name: beta galactosidase Alteration at locus: Transgenic |
Genetic Alterations:
The cell line has LacZ expression under control of a chicken beta actin promoter coupled with CMV immediate early enhancer. The beta-galactosidase gene is flanked by lox P sites and in the presence of cre-recombinase the beta-galactoside is excised and this allows expression of the second reporter contained in this cell line which is DsRed.MST (variant of DsRed).
Genotype Determination:
Parental ES Cell Line: Nagy ES cell line G4 (MMRRC:011986)Mouse strain from which the parental cell line was derived: 129S6/SvEvTac X C57BL/6Ncr F1
Related MMRRC Products:
ACTB: MMRRC:011981-MU Nagy ES cell line R1 with EGFP (B5)
ACTB: MMRRC:011982-MU Nagy ES cell line R1 with EYFP (YC5)
ACTB: MMRRC:011983-MU Nagy ES cell line R1 with EYFP (7AC5)
ACTB: MMRRC:011984-MU Nagy ES cell line R1 with ECFP (CK6)
ACTB: MMRRC:011985-MU Nagy ES cell line R1 with ECFP (CK8)
ACTB: MMRRC:011988-MU Nagy ES cell line G4 with DsRed.MST transgene
ACTB: MMRRC:011991-MU Nagy ES cell line YV1
ACTB: MMRRC:036741-UCD C57BL/6N-Tg(C330027C09Rik-ACTB)1Btk/Mmucd
ACTB: MMRRC:036801-UNC STOCK Tg(ACTB-HistH2b/GFP)1Mboi/Mmnc
Related Protocols:
- Culture conditions for R1 and G4 ES cells (November 2004)
- Nagy A, Vintersten K. Murine embryonic stem cells. Methods Enzymol. 2006;418:3-21. (Medline PMID: 17141026)
Strain Origin
Donor: Andras Nagy, Ph.D., Mt. Sinai Hospital, Toronto
Primary Reference:
- Vintersten K, Monetti C, Gertsenstein M, Zhang P, Laszlo L, Biechele S, Nagy A. Mouse in red: red fluorescent protein expression in mouse ES cells, embryos, and adult animals. Genesis. 2004 Dec;40(4):241-6. Erratum in: Genesis. 2005 Jul;42(3):218. (Medline PMID: 15593332)
- George SH, Gertsenstein M, Vintersten K, Korets-Smith E, Murphy J, Stevens ME, Haigh JJ, Nagy A. Developmental and adult phenotyping directly from mutant embryonic stem cells. Proc Natl Acad Sci U S A. 2007 Mar 13;104(11):4455-60. Epub 2007 Mar 2. (Medline PMID: 17360545)
Special Considerations
Transgenic mice (STOCK Tg (ACTB-Bgeo,DsRed.MST)1Nagy/J; JAX Stock # 005438) have been made from this cell line.
Supplemental Information
Health Status Report
Requested cell lines are tested by PCR analysis for mycoplasma spp. and Murine Parvoviruses (MPV) upon completion of expansion and prior to shipment. Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email mmrrc@missouri.edu.
Order Request Information
Availability Level:
Cryo-preserved ES cell line material can usually be shipped in 2 to 4 weeks. Genetic confirmation adds an additional 4 to 6 weeks (but is recommended to confirm that the material has not been contaminated or otherwise incorrectly identified). The microinjection services add an additional 10 to 12 weeks and can vary from cell line to cell line.
Conditions of Distribution:
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Fees:
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
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Distribution Fee/unit (US $) |
Units | Notes | ||||
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| Vial | Without sequence confirmation.* | ||||
*Genetic confirmation of a cell line is an add-on service; cost is in addition to the cost of the cell line itself. We recommend minimum verification of the cell line by genetic confirmation of the reporter sequence. Please inquire with the supplying MMRRC facility when ordering (mmrrc@missouri.edu).
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-7510 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.
The MMRRC is a collaborative effort, funded by grants from the NIH
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