Strain Name: E14 RP11-88L12 NKX2-5-EmGFP ES cell line
Stock Number: 030473-UCD
Other Names:
Gene Details: (provided by MGI)
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Reporter: EmGFP (Jelly Fish) Name: Emerald Green Fluorescent Protein Alteration at locus: Transgenic (BAC) |
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Transgene: NKX2-5 (Human) Name: NK2 transcription factor related, locus 5 Alteration at locus: Transgenic (BAC) |
Genetic Alterations:
This is an ES cell line, drived from the E14Tg2A.4 mouse ES cell line. Mouse E14 ES cells were electroporated with a modified human BAC for NKX2-5-EmGFP to make the transgenic reporter line. The BAC is based on RP11-88L12, and carries an in-frame P2A-EmGFP insertion, a Zeocin cassette, and a SV40- Neo selection cassette.
Genotype Determination:
Mouse strain from which the parental cell line was derived:
129P2/OlaHsd
Parental ES Cell Line: E14Tg2A.4
Related MMRRC Products:
Please see also:
Related Protocols:
- Media protocol (for both culture and differentiation protocols)
- Culture protocol
- Cardiomyocyte Differentiation protocol
Strain Origin
Donor: Bruce Conklin [M.D.] [Ph.D.], PhD, Gladstone Institute of Cardiovascular Disease,
Primary Reference:
Hsiao EC, Yoshinaga Y, Nguyen TD, Musone SL, Kim JE, Swinton P, Espineda I, Manalac C, deJong PJ, Conklin BR. Marking embryonic stem cells with a 2A self-cleaving peptide: a NKX2-5 emerald GFP BAC reporter. PLoS ONE. 2008 Jul 2;3(7):e2532. (Medline PMID: 18596956)
Special Considerations
This is Clone #2 as described in publication. See also: MMRRC:030440
Health Status Report
Requested cell lines are tested by PCR analysis for mycoplasma spp. and Murine Parvoviruses (MPV) upon completion of expansion and prior to shipment. Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email mmrrc@ucdavis.edu.
Order Request Information
Availability Level:
Cryo-preserved ES cell line material can usually be shipped in 2 to 4 weeks. Genetic confirmation adds an additional 4 to 6 weeks (but is recommended to confirm that the material has not been contaminated or otherwise incorrectly identified). The microinjection services add an additional 10 to 12 weeks and can vary from cell line to cell line.
Conditions of Distribution:
Fees:
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
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Distribution Fee/unit (US $) |
Units | Notes | ||||
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| Vial | Without sequence confirmation.* | ||||
*Genetic confirmation of a cell line is an add-on service; cost is in addition to the cost of the cell line itself. We recommend minimum verification of the cell line by genetic confirmation of the reporter sequence. Please inquire with the supplying MMRRC facility when ordering (mmrrc@ucdavis.edu).
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-7510 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.
The MMRRC is a collaborative effort, funded by grants from the NIH
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