Strain Name:
FVB;129S6-Foxo3tm1.1Rdp/Mmucd
Stock Number:
016132-UCD
Citation ID:
RRID:MMRRC_016132-UCD
Other Names:
Foxo3a, FVB;129S6-Foxo3tm1.1Rdp/Mmcd

Strain Information

Foxo3tm1.1Rdp
Name: forkhead box O3; targeted mutation 1.1, Ronald DePinho
Synonyms: Foxo3a-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Knockout
Foxo3
Name: forkhead box O3
Synonyms: FKHRL1, Fkhr2, 2010203A17Rik, 1110048B16Rik, Foxo3a
Type: Gene
Species: Mouse
Chromosome: 10
Alteration at locus: Knockout
NCBI: 56484
Homologene: 31039
Genetic Alterations
Deletion of the first coding exon, which encodes the start codon and the Nterminal half of the protein and forkhead DNA binding domain.
Genotype Determination
ES Cell Line
TC1/TC-1 derived from 129S6/SvEvTac
Phenotype
Homozygous: Female homozygous mice exhibit a distinctive ovarian phenotype of global follicular activation leading to oocyte, early depletion of functional ovarian follicles, and secondary infertility.

Heterozygous: Same as wild-type.
Mammalian Phenotype Terms
Allelic Composition: Foxo3tm1.1Rdp/Foxo3tm1.1Rdp (Genetic Background: involves: 129S6/SvEvTac * FVB/N )

MeSH Terms
  • Animals
  • Apoptosis
  • Cell Size
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/physiology
  • Female
  • Follicle Stimulating Hormone/blood
  • Follicular Atresia
  • Forkhead Box Protein O1
  • Forkhead Transcription Factors
  • Gene Targeting
  • Granulosa Cells/cytology
  • Granulosa Cells/physiology
  • Humans
  • Infertility, Female
  • Luteinizing Hormone/blood
  • Male
  • Mice
  • Mice, Knockout
  • Oocytes/cytology
  • Oocytes/physiology
  • Ovarian Follicle/growth & development
  • Ovarian Follicle/physiology
  • Ovary/metabolism
  • Ovulation
  • Primary Ovarian Insufficiency/etiology
  • Sexual Maturation
  • Superovulation
  • Transcription Factors/genetics
  • Transcription Factors/physiology
Strain Development
Foxo3a-/+ animals were backcrossed three times to FVB/n females and progeny of these matings that were Cre- were then intercrossed with littermates to generate the experimental cohort.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Apoptosis
  • Cancer
  • Cardiovascular
  • Cell Biology
  • Dermatology
  • Diabetes/Obesity
  • Endocrine Deficiency
  • Hematology
  • Metabolism
  • Models for Human Disease
  • Reproduction
Donor
Ron DePinho, M.D., Dana Farber Cancer Institute
Primary Reference
Castrillon DH, Miao L, Kollipara R, Horner JW, DePinho RA. Suppression of ovarian follicle activation in mice by the transcription factor Foxo3a. Science. 2003 Jul 11;301(5630):215-8. (Medline PMID: 12855809)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
016132-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / $1,545.65
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
016132-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $869.88
Non-Profit / For-Profit
Aliquot Approximate quantity3
016132-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.