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Consomic Donor Genotyping Protocol
Reed DR, Avigdor M, McDaniel AH, Bachmanov AA. QTL for body composition on chromosome 7 detected using a chromosome substitution mouse strain. Obesity (Silver Spring). 2008 Feb;16(2):483-7. (Medline PMID: 18239664).
McDaniel AH, Li X, Tordoff MG, Bachmanov AA, Reed DR. A locus on mouse chromosome 9 (Adip5) affects the relative weight of the gonadal but not retroperitoneal adipose depots. Mammalian Genome. 2006 Nov; 17(11):1078-1092. Epub 2006 Nov 10. (Medline PMID: 17103052).
Lin C, Bosak NP, Nelson TM, Theodorides ML, Smith ZH, Kirkey M, Avigdor M, Gantick B, Khoshnevisan A, Lysenko A, Reed DR, Bachmanov AA. Construction of reciprocal chromosome substitution strains from 129P3/J and C57BL/6ByJ mice. Poster, Mouse Genetics Conference. Poster Abstract 236C. Washington D.C., June 22-25, 2011.
Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.
Female x Male siblings
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.