Strain Name:
B6.FVB(Cg)-Tg(Agtr1a-EGFP)NZ44Gsat/TmilMmucd
Stock Number:
036905-UCD
Citation ID:
RRID:MMRRC_036905-UCD
Other Names:
Agtr1a BAC GFP, Angiotensin receptor type 1a EGFP

Strain Information

EGFP
Name: enhanced green fluorescent protein
Type: Gene
Species: Aequorea victoria (jellyfish)
Chromosome:
Agtr1a
Name: angiotensin II receptor, type 1a
Synonyms: AT1a, Angtr-1a, Agtr-1a, AT1, Agtr1a, 1810074K20Rik, Agt1ar
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 11607
HGNC: HGNC:336
Homologene: 3556
Tg(Agtr1a-EGFP)NZ44Gsat
Name: transgene insertion NZ44, GENSAT Project at Rockefeller University
Synonyms: NZ44
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic (BAC)
Genetic Alterations
An EGFP reporter gene, followed by a polyadenylation sequence, was inserted into BAC clone RP24-63B19 at the initiating ATG codon of the first coding exon of the Agtr1a gene so that EGFP expression is driven by the regulatory sequences of the BAC gene. The resulting modified BAC (BX2685) was used to generate this transgene.
Phenotype

Homozygous: Expressed GFP in cells containing native angiotensin receptor (AT) type 1a

Hemizygous: Expressed GFP in cells containing native angiotensin receptor (AT) type 1a

MeSH Terms
  • Animals
  • Arginine Vasopressin/immunology
  • Arginine Vasopressin/metabolism
  • Autonomic Nervous System/cytology
  • Autonomic Nervous System/metabolism
  • Brain/cytology
  • Brain Chemistry/genetics
  • Chromosomes, Artificial, Bacterial/genetics
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/immunology
  • Humans
  • Image Processing, Computer-Assisted
  • Immunoenzyme Techniques
  • Immunohistochemistry
  • Mice
  • Mice, Transgenic
  • Microscopy, Electron
  • Microscopy, Immunoelectron
  • RNA, Messenger/biosynthesis
  • RNA, Messenger/genetics
  • Reactive Oxygen Species/metabolism
  • Receptor, Angiotensin, Type 1/metabolism
  • Tyrosine 3-Monooxygenase/metabolism
  • Water-Electrolyte Balance/genetics
  • Water-Electrolyte Balance/physiology
Strain Development
This transgenic mice was generated from GENSAT (Founder NZ44; BAC address RP24-63B19). The transgenic mice have a modified BAC-EGFP injected into pronuclei of FVB/N fertilized oocytes. Hemizygous progeny was mated to IcrTac:ICR mice. Dr. Milner's lab purchased this strain from GENSAT and backcrossed it to C57BL/6 for 6 generations.
MMRRC currently maintains the STOCK strain MMRRC:033059, STOCK Tg(Agtr1a-EGFP)NZ44Gsat/Mmucd for this mutation.

Suggested Control Mice
Wild-type littermates or C57BL/6 mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Diabetes
  • Endocrine Deficiency
  • Immunology and Inflammation
  • Metabolism
  • Models for Human Disease
  • Neurobiology
  • Reproduction
  • Research Tools
Donor
Teresa Milner, Ph.D., Weill Cornell Medical College.
Primary Reference

Gonzalez AD, Wang G, Waters EM, Gonzales KL, Speth RC, Van Kempen TA, Marques-Lopes J, Young CN, Butler SD, Davisson RL, Iadecola C, Pickel VM, Pierce JP, Milner TA. Distribution of angiotensin type 1a receptor-containing cells in the brains of bacterial artificial chromosome transgenic mice. Neuroscience. 2012 Dec 13;226:489-509 (Medline PMID: 22922351).

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
MMRRC Breeding System
Backcross
Breeding Scheme(s)

Wild-type (C57BL/6) female x Hemizygous male or reciprocal mating

Generation
N6+
Overall Breeding Performance
Excellent
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
5-9
Recommended wean age
3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
036905-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
036905-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
036905-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.