Strain Name:
B6;129S4-Mns1tm1Jw/Mmjax
Stock Number:
037095-JAX
Citation ID:
RRID:MMRRC_037095-JAX
Other Names:
MNS1 

Strain Information

Mns1tm1Jw
Name: meiosis-specific nuclear structural protein 1; targeted mutation 1, Jeremy Wang
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Knockout
Mns1
Name: meiosis-specific nuclear structural protein 1
Type: Gene
Species: Mouse
Chromosome: 9
Alteration at locus: Knockout
NCBI: 17427
Homologene: 40628
Genetic Alterations
Exons 3 - 8 were replaced with a floxed neo cassette via homologous recombination on the Mns1 gene. 
Genotype Determination
ES Cell Line
v6.5 derived from (C57BL/6 x 129S4/SvJae)F1
Phenotype

Homozygous: MNS1–deficient males are sterile, as they exhibit a sharp reduction in sperm production and the remnant sperm are immotile with abnormal short tails. In MNS1–deficient sperm flagella, the characteristic arrangement of ‘‘9+2’’ microtubules and outer dense fibers are completely disrupted. In addition, MNS1–deficient mice display situs inversus and hydrocephalus. MNS1–deficient tracheal motile cilia lack some outer dynein arms in the axoneme.

Heterozygous: No observable phenotype.

Mammalian Phenotype Terms
Allelic Composition: Mns1tm1Jw/Mns1tm1Jw (Genetic Background: involves: 129S4/SvJae * C57BL/6 )

MeSH Terms
  • Animals
  • Cells, Cultured
  • Cilia/genetics
  • Cilia/physiology
  • Fertility/genetics
  • Germ Cells/cytology
  • Germ Cells/metabolism
  • Hydrocephalus/genetics
  • Male
  • Mice
  • Nuclear Proteins/deficiency
  • Nuclear Proteins/genetics
  • Nuclear Proteins/metabolism
  • Sperm Tail/metabolism
  • Spermatids/cytology
  • Spermatids/growth & development
  • Spermatogenesis/genetics
  • Spermatogenesis/physiology
  • Spermatozoa/cytology
  • Spermatozoa/growth & development
  • Trachea/cytology
  • Trachea/metabolism
Strain Development
V6.5 ES cells derived from (C57BL/6 x 129S4/SvJae)F1 were electroporated with linearized targeting construct. Correctly targeted ES cells were injected into B6C3F1/Tac blastocysts. Chimeric mice were bred to C57BL/6J mice to confirm germline transmittion of the the Mns1 mutant allele.
Suggested Control Mice
Wild-type littermates or C57BL/6 mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
    • Atherosclerosis
    • Rheumatoid arthritis
    • Breast cancer
  • Reproduction
Donor
P. Jeremy Wang, Ph.D., University of Pennsylvania.
Primary Reference

Zhou J, Yang F, Leu NA, Wang PJ. MNS1 is essential for spermiogenesis and motile ciliary functions in mice. PLoS Genet. 2012;8(3):e1002516. Epub 2012 Mar 1. (Medline PMID:22396656)

Colony and Husbandry Information

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
Black or Agouti
MMRRC Breeding System
Backcross or Intra-strain Random Mating
Breeding Scheme(s)
  • Backcross:
    • Wild-type (STRAIN) female x Heterozygous male or reciprocal mating
  • Intra-strain Random Mating
    • Wild-type female x Heterozygous male from the colony or reciprocal mating
Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Sterile
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Less than 4 months
Average litter size
5-9
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
037095-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
037095-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.