Strain Name:
129S6;129S8-Gnai3tm1Lbi Gnai1tm1Lbi/Mmjax
Stock Number:
037344-JAX
Citation ID:
RRID:MMRRC_037344-JAX
Other Names:
Galpha-i1/Galpha-i3 DKO, Gi1/Gi3 DKO

Strain Information

Gnai3tm1Lbi
Name: G protein subunit alpha i3; targeted mutation 1, Lutz Birnbaumer
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Knockout
Gnai1tm1Lbi
Name: G protein subunit alpha i1; targeted mutation 1, Lutz Birnbaumer
Synonyms: Gnao1.1tm1LBi
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Knockout
Gnai3
Name: G protein subunit alpha i3
Synonyms: Galphai3
Type: Gene
Species: Mouse
Chromosome: 3
Alteration at locus: Knockout
NCBI: 14679
HGNC: HGNC:4387
Homologene: 55975
Gnai1
Name: G protein subunit alpha i1
Synonyms: Gialpha1
Type: Gene
Species: Mouse
Chromosome: 5
Alteration at locus: Knockout
NCBI: 14677
HGNC: HGNC:4384
Homologene: 74417
Genetic Alterations
Part of intron 5 and exon 6 were replaced with a neo cassette on the Gnai3 gene and a neo cassette was inserted into exon 3 on the Gnai1 gene. 
ES Cell Line
CCE/EK.CCE

Phenotype

Homozygous: Exhibit fusion of ribs and lumbar vertebrae. 

Heterozygous: No observable phenotype.

Mammalian Phenotype Terms
Allelic Composition: Gnai3tm1Lbi/Gnai3tm1Lbi (Genetic Background: B6.129S-Gnai3tm1Lbi )

Allelic Composition: Gnai3tm1Lbi/Gnai3tm1Lbi (Genetic Background: involves: 129S/SvEv )

Allelic Composition: Gnai3tm1Lbi/Gnai3tm1Lbi (Genetic Background: involves: 129S/SvEv * C57BL/6 )

Allelic Composition: Gnai1tm1Lbi/Gnai1+ (Genetic Background: involves: 129S/Sv )

MeSH Terms
  • Animals
  • Brain/drug effects
  • Brain/metabolism
  • Brain/pathology
  • Dopamine D2 Receptor Antagonists
  • GTP-Binding Protein alpha Subunits, Gi-Go/metabolism
  • Guanosine 5'-O-(3-Thiotriphosphate)/agonists
  • Guanosine 5'-O-(3-Thiotriphosphate)/metabolism
  • Heterotrimeric GTP-Binding Proteins/genetics
  • Heterotrimeric GTP-Binding Proteins/metabolism
  • Mice
  • Mice, Knockout
  • Receptors, Dopamine D2/metabolism
  • Sulpiride/analogs & derivatives
  • Sulpiride/pharmacology
Strain Development
The mouse strain was developed by breeding individual knockout mice carrying single mutated alleles for Gnai1 and Gnai3 to generate mice carrying both mutated alleles. Prior, both parental strains were made from correctly targeted CCE/EK.CCE ES cells derived from 129S8/SvEv-Gpi1c injected into blastocysts to generate chimeric mice. Chimeric mice from the individual knockout strains were then bred to 129S6/SvEvTac mice for several generations. Double-knockouts maintained as homozygotes.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Apoptosis
  • Cell Biology
  • Developmental Biology
  • Endocrine Deficiency
  • Internal Organ
  • Metabolism
  • Reproduction
Donor
Lutz Birnbaumer, Ph.D., National Institute of Environmental Health Sciences.
Primary Reference

Jiang M, Spicher K, Boulay G, Wang Y, Birnbaumer L. Most central nervous system D2 dopamine receptors are coupled to their effectors by Go. Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3577-82. (Medline PMID: 11248120)

Colony and Husbandry Information

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
Agouti
Eye
Black
MMRRC Breeding System
Sib-mating
Breeding Scheme(s)

Homozygous female x Homozygous male littermate

Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Bred to Homozygosity
Yes
Average litter size
4-6
Recommended wean age
3 weeks
Average Pups Weaned
4-6

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
037344-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
037344-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.