Strain Name:
B6.Cg-Tg(CD2-Tcra,Cd4-Tcrb)4CKang/Mmucd
Stock Number:
037367-UCD
Citation ID:
RRID:MMRRC_037367-UCD
Other Names:
4C T cell receptor transgenic, 4C

Strain Information

Cd4
Name: CD4 antigen
Synonyms: L3T4, Ly-4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12504
HGNC: HGNC:1678
Homologene: 513
Tcrb
Name: T cell receptor beta chain
Synonyms: Tib, TCRbeta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Cd2
Name: CD2 antigen
Synonyms: LFA-2, Ly37, Ly-37
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 12481
HGNC: HGNC:1639
Homologene: 1338
Tcra
Name: T cell receptor alpha chain
Synonyms: Tcr alpha, Tcralpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Tg(CD2-Tcra,Cd4-Tcrb)4CKang
Name: transgene insertion 4C, Sang-Mo Kang
Synonyms: 4C TCR
Type: Transgene
Species: Mus musculus (mouse)
Chromosome:
Alteration at locus: Transgenic
Genetic Alterations
This transgene results from the coinjection of two constructs derived from a C57BL/6 hybridoma cell line responsive to BALB/c splenocytes. The Tcra construct under the control of the human CD2 promoter contains a cDNA including V alpha 8.5 sequence and the Tcrb construct under the control of the mouse Cd4 promoter (without the intronic silencer) contains a cDNA including V beta 13 sequence.
Genotype Determination
ES Cell Line
NA
Phenotype

Homozygous: Neurologically impaired as reaches adulthood.

Heterozygous: No observable phenotype.

MeSH Terms
  • Animals
  • Bone Marrow Cells/immunology
  • CD4 Antigens/immunology
  • DNA Primers
  • Dendritic Cells/immunology
  • Graft Rejection/immunology
  • Histocompatibility Antigens Class II/genetics
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Polymerase Chain Reaction
  • Receptors, Antigen, T-Cell/genetics
  • Adoptive Transfer
  • CD4-Positive T-Lymphocytes/immunology
  • CD8-Positive T-Lymphocytes/immunology
  • Enzyme-Linked Immunosorbent Assay
  • Flow Cytometry
  • Genes, T-Cell Receptor alpha/immunology
  • Heart Transplantation/immunology
  • Immunologic Memory
  • Interferon-gamma/immunology
  • Interleukin-2/immunology
  • Lymphocytes/immunology
  • Skin Transplantation/immunology
  • T-Lymphocytes/immunology
  • Thy-1 Antigens/immunology
  • Transplantation, Homologous/immunology
  • Cell Proliferation
  • Graft Survival/immunology
  • Immunocompetence/immunology
  • Immunomodulation
  • Models, Animal
  • T-Lymphocytes, Regulatory/immunology
  • T-Lymphocytes, Regulatory/transplantation
  • Transplantation Tolerance/immunology
  • Transplantation, Homologous
  • Dendritic Cells
  • Female
  • Graft vs Host Disease/etiology
  • Graft vs Host Disease/mortality
  • Hematologic Neoplasms/complications
  • Hematologic Neoplasms/therapy
  • Heparitin Sulfate/adverse effects
  • Humans
  • Luciferases/metabolism
  • Lymphocyte Depletion
  • Male
  • Middle Aged
  • Stem Cell Transplantation/adverse effects
  • Survival Rate
  • T-Lymphocytes/drug effects
  • Toll-Like Receptor 4/agonists
  • CD4 Antigens/metabolism
  • Cytokines/immunology
  • Fetal Therapies
  • Fetus/immunology
  • Histocompatibility, Maternal-Fetal/immunology
  • Immune Tolerance/immunology
  • Interleukin-2 Receptor alpha Subunit/metabolism
  • Lymphocyte Activation/immunology
  • Maternal-Fetal Exchange/immunology
  • Pregnancy
  • Pregnancy Complications/immunology
  • T-Lymphocyte Subsets/immunology
  • Uterus/immunology
  • Antigen Presentation/immunology
  • Antigen-Presenting Cells/cytology
  • Antigen-Presenting Cells/immunology
  • Cell Survival/immunology
  • Fetal Death/immunology
  • Fetal Diseases/immunology
  • Fetal Diseases/therapy
  • Hematopoietic Stem Cell Transplantation/methods
  • Lymphocyte Culture Test, Mixed
  • T-Lymphocytes, Regulatory/cytology
  • Thymus Gland/cytology
  • Thymus Gland/immunology
  • Transplantation Chimera/immunology
  • Bone Marrow Cells/cytology
  • Bone Marrow Transplantation
  • CD4-Positive T-Lymphocytes/cytology
  • Cell Communication/immunology
  • Dendritic Cells/cytology
  • Heart Transplantation
  • Killer Cells, Natural/cytology
  • Killer Cells, Natural/immunology
  • Mice, Knockout
  • Microscopy, Fluorescence, Multiphoton
  • Transplantation Immunology
Strain Development
Transgenic mice were then generated by pronuclear coinjection of constructs into (C57BL/6 x DBA/2)F2 eggs. Founder 4C expressing TCR V13 on 99% of peripheral CD4 T cells was then backcrossed into the C57BL/6 Rag1 knockout Ly5.1 background 9 generations using Speed Congenics for the first 5 generations. Strain was then backcrossed for more than 10 generations on the B6.Cg-Thy1a background, including 5 rounds of speed genomics.

This TCR-tg mouse model may be useful in adoptive transfer experiments for studying various aspects of direct alloantigen presentation, such as studies of alloimmunity, including transplantation, graft-vs-host disease, and maternal-fetal immune tolerance.
Suggested Control Mice
Wild-type littermates or B6.Cg-Thy1a mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cell Biology
  • Immunology and Inflammation
  • Models for Human Disease
    • Transplant organ rejection
    • Graft vs Host disease
  • Research Tools
Donor
Todd Brennan, M.D., Duke University Medical Center.
Primary Reference
  • Brennan TV, Hoang V, Garrod KR, Liu FC, Hayden T, Kim J, Kang SM. A new T-cell receptor transgenic model of the CD4+ direct pathway: level of priming determines acute versus chronic rejection. Transplantation. 2008 Jan 27;85(2):247-55. (Medline PMID: 18212630)
  • Brennan TV, Jaigirdar A, Hoang V, Hayden T, Liu FC, Zaid H, Chang CK, Bucy RP, Tang Q, Kang SM. Preferential priming of alloreactive T cells with indirect reactivity. Am J Transplant. 2009 Apr;9(4):709-18. (Medline PMID: 19344462)
  • Brennan TV, Tang Q, Liu FC, Hoang V, Bi M, Bluestone JA, Kang SM. Requirements for prolongation of allograft survival with regulatory T cell infusion in lymphosufficient hosts. J Surg Res. 2011 Jul;169(1):e69-75. (Medline PMID: 21571317)
  • Brennan TV, Lin L, Huang X, Cardona DM, Li Z, Dredge K, Chao NJ, Yang Y. Heparan sulfate, an endogenous TLR4 agonist, promotes acute GVHD after allogeneic stem cell transplantation. Blood. 2012 Oct 4;120(14):2899-908. (Medline PMID: 22760779)
  • Wegorzewska M, Nijagal A, Wong CM, Le T, Lescano N, Tang Q, MacKenzie TC. Fetal intervention increases maternal T cell awareness of the foreign conceptus and can lead to immune-mediated fetal demise. J Immunol. 2014 Feb 15;192(4):1938-45.(Medline PMID:24415782)
  • Nijagal A, Derderian C, Le T, Jarvis E, Nguyen L, Tang Q, Mackenzie TC. Direct and indirect antigen presentation lead to deletion of donor-specific T cells after in utero hematopoietic cell transplantation in mice. Blood. 2013 May 30;121(22):4595-602. (Medline PMID:23610372)
  • Garrod KR, Liu FC, Forrest LE, Parker I, Kang SM, Cahalan MD. NK cell patrolling and elimination of donor-derived dendritic cells favor indirect alloreactivity. J Immunol. 2010 Mar 1;184(5):2329-36. (Medline PMID:20139277)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
MMRRC Breeding System
Backcross or Sib-mating
Breeding Scheme(s)
  • Backcross:
    • Wild-type (B6.Cg-Thy1a) female x Heterozygous male or reciprocal mating
  • Sib-mating:
    • Wild-type female x Heterozygous male littermate or reciprocal mating
Generation
N20
Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: Undetermined Undetermined
Homozygotes are fertile: Undetermined Undetermined
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: >12 months >12 months
Average litter size
5-9
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
037367-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
037367-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
037367-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.