Strain Name:
FVB/N-Tg(Figla-EGFP,-icre)ZP3Dean/Mmjax
Stock Number:
037373-JAX
Citation ID:
RRID:MMRRC_037373-JAX
Other Names:
Figla/EGFP/Cre, FEIC

Strain Information

Figla
Name: folliculogenesis specific basic helix-loop-helix
Synonyms: FIG alpha, bHLHc8
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 26910
Homologene: 49294
EGFP
Name: enhanced green fluorescent protein
Type: Gene
Species: Aequorea victoria (jellyfish)
Chromosome:
icre
Name: codon-improved cre recombinase sequence
Type: Gene
Species: Enterobacteria phage P1 (bacteriophage P1)
Chromosome:
Tg(Figla-EGFP,-icre)ZP3Dean
Name: transgene insertion ZP3, Jurrien Dean
Synonyms: Figla-EGFP/Cre
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
FIGLA (folliculogenesis specific basic helix-loop-helix) is a basic helix-loop-helix transcription factor that regulates multiple oocyte-specific genes. These transgenic mice express EGFP and Cre recombinase under the control of the Figla promoter beginning at E14.5 in female germ cells. EGFP also is observed in the cytoplasm of male germ cells throughout all stages of spermatogenesis. These mice may be useful for the study of folliculogenesis.

ES Cell Line
NA
Phenotype
FIGLA (folliculogenesis specific basic helix-loop-helix) is a basic helix-loop-helix transcription factor that regulates multiple oocyte-specific genes. These transgenic mice express EGFP and Cre recombinase under the control of the Figla promoter beginning at E14.5 in female germ cells. EGFP also is observed in the cytoplasm of male germ cells throughout all stages of spermatogenesis. These mice may be useful for the study of folliculogenesis.
Strain Development
A transgenic construct containing sequence encoding an EGFP-IRES-iCRE cassette under the control of the folliculogenesis specific basic helix-loop-helix (Figla) promoter was introduced into FVB/N fertilized mouse eggs. Founder line ZP3 was subsequently established and crossed to a FVB/N male by the donating laboratory. Upon arrival to The Jackson Laboratory, mice were bred to FVB/NJ to establish the colony.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Developmental Biology
  • Models for Human Disease
    • Infertility
  • Reproduction
Donor
Jurrien Dean, M.D., National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health.

Colony and Husbandry Information

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
White
Other
Very small in body
MMRRC Breeding System
Backcross or Sib-mating
Breeding Scheme(s)
  • Backcross
    • Wild-type (FVB/N) female x Hemizygous male or reciprocal mating
  • Sib-mating
    • Wild-type female x Hemizygous male littermate or reciprocal mating
    • Homozygous female x Homozygous male littermate
Generation
N3
Overall Breeding Performance
Poor
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: 6 months 10 months
Average litter size
5-9
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
037373-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
037373-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.