Strain Name:
B6NTac.129S4(Cg)-Ccr1tm1Gao/Mmmh
Stock Number:
037392-MU
Citation ID:
RRID:MMRRC_037392-MU

Strain Information

Ccr1tm1Gao
Name: C-C motif chemokine receptor 1; targeted mutation 1, Ji-Liang Gao
Synonyms: CCR1-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Knockout
Ccr1
Name: C-C motif chemokine receptor 1
Synonyms: Cmkbr1
Type: Gene
Species: Mouse
Chromosome: 9
Alteration at locus: Knockout
NCBI: 12768
VEGA: 9
HGNC: HGNC:1602
Homologene: 20344
Genetic Alterations
A neomycin resistance cassette replaced open reading frame sequences that correspond to 33% of the protein, from the third to the fifth transmembrane segment. Transcript was not detected in neutrophils of homozygous mutant animals.
Genotype Determination
ES Cell Line
J1 derived from 129S4/SvJae
Phenotype
The initial analysis indicated that the Ccr1 Knockout mouse has impaired hematopoiesis, host defense, and inflammatory responses. The knockout mouse also has alterations in the type 1-type 2 cytokine balance.
Mammalian Phenotype Terms
Allelic Composition: Ccr1tm1Gao/Ccr1tm1Gao (Genetic Background: B6.129S4-Ccr1tm1Gao )

Allelic Composition: Ccr1tm1Gao/Ccr1tm1Gao (Genetic Background: involves: 129S4/SvJae )

Allelic Composition: Ccr1tm1Gao/Ccr1tm1Gao (Genetic Background: involves: 129S4/SvJae * C57BL/6 )

Allelic Composition: Ccr1tm1Gao/Ccr1tm1Gao (Genetic Background: involves: C57BL/6 )

MeSH Terms
  • Animals
  • Aspergillosis/immunology
  • Aspergillus fumigatus
  • Calcium/metabolism
  • Cell Division
  • Chemokine CCL3
  • Chemokine CCL4
  • Chemotaxis, Leukocyte
  • Cytokines/metabolism
  • Gene Targeting
  • Granuloma/immunology
  • Hematopoiesis
  • Hematopoietic Stem Cells/physiology
  • Interferon-gamma/metabolism
  • Interleukin-4/metabolism
  • Macrophage Inflammatory Proteins/pharmacology
  • Macrophages/metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Mutagenesis/genetics
  • Neutrophils/immunology
  • Receptors, CCR1
  • Receptors, Chemokine
  • Receptors, Cytokine/deficiency
  • Receptors, Cytokine/genetics
  • Receptors, Cytokine/physiology
  • Schistosomiasis mansoni/immunology
Strain Development
Ccr1 gene was disrupted in 129S4/SvJae-derived J1 ES cells. Resultant chimeras were backcrossed to C57BL/6NAi mice for 10 generations in the NIAID barrier facility at Taconic. At this point, it was discovered that the C57NL/6NAi line had become contaminated with IFNg knockout gene. Line 4087 was then backrossed an additional 5 times to C57BL/6NTac in order to eliminate the contaminating gene and put the strain on the Taconic C57BL/6 subline.
Suggested Control Mice
Wild-type littlemates or C57BL/6NTac mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Immunology and Inflammation
Donor
Ji-Liang Gao, National Institute of Allergy and Infectious Diseases.
Primary Reference
Gao J-L, Wynn TA, Chang Y, Lee E, Broxmeyer HE, Cooper S, Tiffany HL, Westphal H, Kwon-Chung J, and Murphy PM. Impaired host defense, hematopoiesis, granulomatous inflammation and type 1-type 2 cytokine balance in mice lacking CCR1. J Exp Med. 1997 Jun 2;185(11):1959-68. (Medline PMID: 9166425)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
Breeding Scheme(s)
  • Homozygous x Homozygous
  • Wild-type (C57BL/6NTac) female x Heterozygous male or reciprocal mating
Generation
N10+N5
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
037392-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.