Strain Name:
B6N.Cg-Tg(Cd4-cre)1Cwi/TacMmmh
Stock Number:
037393-MU
Citation ID:
RRID:MMRRC_037393-MU

Strain Information

cre
Name: cre recombinase
Type: Gene
Species: Enterobacteria phage P1 (bacteriophage P1)
Chromosome:
Cd4
Name: CD4 antigen
Synonyms: L3T4, Ly-4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12504
HGNC: HGNC:1678
Homologene: 513
Tg(Cd4-cre)1Cwi
Name: transgene insertion 1, Christopher B Wilson
Synonyms: CD4p-Cre, CD4-Cre+, CD4CRE, CD4-Cre, CD4Cre, CD4-creTg, CD4cre, Tg(CD4-cre)1Cw1
Type: Transgene
Species: Multi-species
Chromosome: unknown
Alteration at locus: Transgenic (cre)
Genetic Alterations
Cre recombinase is expressed under the control of mouse Cd4 regulatory elements. The Cd4 enhancer, promoter and silencer collectively drive expression at sequential stages specifically of CD4+ T cell development.
Genotype Determination
Phenotype
Cd4 enhancer/promoter/silencer, becomes active at sequential stages of T cell development. Cre mRNA expression was restricted to lymphoid tissues of these mice.
MeSH Terms
  • Animals
  • Cell Differentiation/genetics
  • Cell Differentiation/immunology
  • Cell Lineage/genetics
  • Cell Lineage/immunology
  • DNA (Cytosine-5-)-Methyltransferase 1
  • DNA (Cytosine-5-)-Methyltransferases/genetics
  • DNA (Cytosine-5-)-Methyltransferases/immunology
  • DNA Methylation
  • Gene Expression Regulation/immunology
  • Mice
  • Mice, Transgenic
  • T-Lymphocytes/immunology
Strain Development
The Cd4-cre mouse was created by pronuclear injection of the Cd4-cre construct into B6D2F2 fertilized eggs. The founder line was first crossed three times to the C57BL/6J strain, and then bred to produce homozygotes. Later they were crossed to mice of a mixed B6;129Sv/J background. The resultant STOCK-Tg(Cd4-cre) mice were screened for H-2 expression and offspring homozygous for H-2b were selected for breeding. Homozygous mice maintained on this mixed background were then transferred to the NIAID repository at Taconic for embryo rederivation by crossing with C57BL/6NTac mice. The derived mice were crossed one additional generation to C57BL/6NAi mice. PCR analysis was used to confirm that the derived pups carried the Cd4-cre transgene. These pups were then backcrossed to C57BL/6NTac from N4 to N9. The line was then intracrossed to homozygosity.
Suggested Control Mice
Wild-type littermates or C57BL/6NTac mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Immunology and Inflammation
Donor
Chris Wilson, Ph.D., University of Washington
Primary Reference
Lee PP, Fitzpatrick DR, Beard C, Jessup HK, Lehar S, Makar KW, Perez-Melgosa M, Sweetser MT, Schlissel MS, Nguyen S, Cherry SR, Tsai JH, Tucker SM, Weaver WM, Kelso A, Jaenisch R, Wilson CB. A critical role for Dnmt1 and DNA methylation in T cell development, function, and survival. Immunity. 2001 Nov;15(5):763-74. (Medline PMID: 11728338)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Intra-strain Random Mating
Breeding Scheme(s)
  • Homozygous female x Homozygous male from the colony
Generation
N9
Overall Breeding Performance
Not specified
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Not specified Not specified
Average litter size
Not specified
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
037393-MU-RESUS Litter recovered from cryo-archive $2,016.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.