Strain Name:
STOCK Gt(ROSA)26Sortm1.1(birA)Mejr Zfpm2tm1.1Wtp/Mmjax
Stock Number:
037509-JAX
Citation ID:
RRID:MMRRC_037509-JAX
Other Names:
Fog2-flbio
Major Collection:

Strain Information

Gt(ROSA)26Sortm1.1(birA)Mejr
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Dies Meijer
Synonyms: bira, Rosa26BirA
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Gene Trap
Zfpm2tm1.1Wtp
Name: zinc finger protein, multitype 2; targeted mutation 1.1, William T Pu
Synonyms: CVDC/B2B program mutation, Fog2-flbio
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Knockout
Zfpm2
Name: zinc finger protein, multitype 2
Synonyms: FOG-2, FOG2, B330005D23Rik
Type: Gene
Species: Mouse
Chromosome: 15
Alteration at locus: Knockout
NCBI: 22762
VEGA: 15
Homologene: 8008
Genetic Alterations
  • Zfpm2tm1.1WtpA targeting vector was designed to insert a FLAG/bio epitope tag and a frt-flanked neomycin resistance (neo) cassette at the endogenous Zfpm2 stop codon and upstream of the 3' UTR. 
  • Gt(ROSA)26Sortm1.1(birA)Mejr: Created using an HA (hemagglutinin) tagged bacterial birA, bifunctional protein (biotin ligase), gene inserted into exon 3 of the rabbit beta-globin gene.
  • The entire birA ORF, with the rabbit beta globin splice acceptor and transcription termination sequences were excised to generate a targeting vector that also contained a floxed PGK- puromycin selection cassette. This targeting vector was inserted into the first intron of the Gt(ROSA)26Sor gene.
ES Cell Line

ES Cells for each allele:

  • Zfpm2tm1.1Wtp - J1 derived from 129S4/SvJae
  • Gt(ROSA)26Sortm1.1(birA)Mejr - IB10 derived from 129P2/OlaHsd
Phenotype
These biallelic mutant mice possess a FLAG/bio epitope tag downstream of the 3' UTR of Zfpm2. ZFPM2 (Zinc finger protein, multitype 2, also called FOG2) is a zinc finger transcription factor expressed in the embryonic heart, septum transversum, developing neuroepthelium and urogenital ridge. In the adult, ZPFM2 is expressed predominantly in heart, brain and testis. ZFPM2 modulates the activity of GATA family proteins. These mice also contain the Gt(ROSA)26Sortm1.1(birA)Mejr allele, which expresses HA (hemagglutinin) tagged bacterial birA, bifunctional protein (biotin ligase). Mice homozygous for both alleles are viable and fertile.
Mammalian Phenotype Terms
Allelic Composition: Zfpm2tm1.1Wtp/Zfpm2tm1.1Wtp (Genetic Background: involves: 129S4/SvJae * C57BL/6 * SJL * Swiss Webster )

  • normal phenotype
MeSH Terms
  • Animals
  • Biotinylation/methods
  • Carbon-Nitrogen Ligases/genetics
  • Carbon-Nitrogen Ligases/metabolism
  • Escherichia coli Proteins/genetics
  • Escherichia coli Proteins/metabolism
  • Female
  • GATA1 Transcription Factor/genetics
  • GATA1 Transcription Factor/metabolism
  • Genetic Vectors
  • Liver/cytology
  • Liver/embryology
  • Liver/metabolism
  • Mice
  • Mice, Transgenic
  • Octamer Transcription Factor-6/genetics
  • Octamer Transcription Factor-6/metabolism
  • Proteins/genetics
  • RNA, Untranslated
  • Repressor Proteins/genetics
  • Repressor Proteins/metabolism
  • Schwann Cells/metabolism
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Transcription, Genetic
  • Transfection
Strain Development
Zfpm2tm1.1Wtp: The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells and correctly targeted ES cells were injected into blastocysts. The resulting chimeric mice were bred to outbred Swiss Webster mice. Offspring were bred with Tg(ACTFLPe)9205Dym transgenic mice to delete the neo cassette. The resulting Zfpm2 (or Fog2)-flbio mice were maintained on a Swiss Webster background.
  • Gt(ROSA)26Sortm1.1(birA)Mejr: The construct was electroporated into 129P2/OlaHsd derived IB10 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to FVB/N mice. The mice were intercrossed for 3 generations before being crossed to a Cre recombinase expressing strain (on an unknown genetic background) to remove the loxP-flanked PGK-puromycin selection cassette. A single loxP site remains. The Cre transgene was removed by breeding.
  • Suggested Control Mice
    None
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
    • Research Tool
    Donor
    William Pu, M.D., Ph.D, Children's Hospital Boston, Harvard Medical School. CvDC Bench to Bassinet.
    Primary Reference
    Driegen S, Ferreira R, van Zon A, Strouboulis J, Jaegle M, Grosveld F, Philipsen S, Meijer D. A generic tool for biotinylation of tagged proteins in transgenic mice. Transgenic Res. 2005 Aug;14(4):477-82. (Medline PMID: 16201414)

    Colony and Husbandry Information

    Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
    MMRRC Breeding System
    Intra-strain random mating
    Breeding Scheme(s)
    • Homozygous female x Homozygous male from within the colony or reciprocal mating
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Undetermined Undetermined
    Average litter size
    Undetermined
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    037509-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
    037509-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.