Strain Name:
B6;129S7-Hoxa13tm1Jwi/Mmnc
Stock Number:
037520-UNC
Citation ID:
RRID:MMRRC_037520-UNC
Other Names:
HOXA13 (Alanine expansion 10), Hoxa13Ala28

Strain Information

Hoxa13tm1Jwi
Name: homeobox A13; targeted mutation 1, Jeffrey W Innis
Synonyms: Hoxa13Ala28
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Targeted Mutation
Hoxa13
Name: homeobox A13
Synonyms: Hox-1.10
Type: Gene
Species: Mouse
Chromosome: 6
Alteration at locus: Targeted Mutation
NCBI: 15398
HGNC: HGNC:5102
Homologene: 73882
Genetic Alterations
Homologous recombination was used to insert 10 additional alanine residues into repeat III, bringing the largest polyalanine sequence up to 28 residues. Transient Cre expression was used to remove the PGK-Neo cassette.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    AB1 derived from 129S7/SvEvBrd-Hprt+
    Phenotype

    Homozygous: Prenatal lethal.

    Heterozygous: Shortening of the first digit or nail of the hind foot and occasional cutaneous syndactyly between digits two and three.

    Mammalian Phenotype Terms
    Allelic Composition: Hoxa13tm1Jwi/Hoxa13tm1Jwi (Genetic Background: involves: 129S7/SvEvBrd )

    Allelic Composition: Hoxa13tm1Jwi/Hoxa13+ (Genetic Background: involves: 129S7/SvEvBrd )

    MeSH Terms
    • Animals
    • Base Sequence
    • Embryo, Mammalian/cytology
    • Female
    • Foot Deformities, Congenital/genetics
    • Hand Deformities, Congenital/genetics
    • Homeodomain Proteins/genetics
    • Homeodomain Proteins/metabolism
    • Humans
    • Male
    • Mice
    • Mice, Mutant Strains
    • Molecular Sequence Data
    • Pedigree
    • Peptides/genetics
    • Protein Biosynthesis
    • Protein Denaturation
    • RNA Splicing
    • RNA, Messenger/genetics
    • Recombination, Genetic
    • Stem Cells/cytology
    • Stem Cells/metabolism
    • Syndrome
    • Trinucleotide Repeats
    Strain Development
    Correctly targeted ES cells derived from 129S7/SvEvBrd-Hprt+ lacking the neomycin resistance cassette were injected into C57BL/6J blastocysts to create chimeric mice. Chimeric male mice were mated with C57BL/6J females to generate agouti offspring. Agouti offspring were then backcrosssed with C57BL/6J mice for more than 3 generations.
    Suggested Control Mice
    Wild-type littermates or C57BL/6J mice
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Cancer
    • Cell Biology
    • Developmental Biology
    • Hematology
    • Immunology and Inflammation
    • Internal Organ
    • Metabolism
    • Models for Human Disease
      • Hand-foot-genital syndrome
    • Reproduction
    • Research Tools
    Donor
    Jeffrey Innis, M.D., Ph.D., University of Michigan.
    Primary Reference

    Innis JW, Mortlock D, Chen Z, Ludwig M, Williams ME, Williams TM, Doyle CD, Shao Z, Glynn M, Mikulic D, Lehmann K, Mundlos S, Utsch B. Polyalanine expansion in HOXA13: three new affected families and the molecular consequences in a mouse model. Hum Mol Genet. 2004 Nov 15;13(22):2841-51. (Medline PMID:15385446)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
    Coat Color
    Agouti
    MMRRC Breeding System
    Backcross
    Breeding Scheme(s)

    Wild-type (C57BL/6J) female x Heterozygous male or reciprocal mating

    Generation
    N3+
    Overall Breeding Performance
    Excellent
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: No No
    Homozygotes are fertile: No No
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Undetermined Undetermined
    Average litter size
    5-9
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    037520-UNC-EMBRYO Cryo-preserved embryos $1,404.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
    037520-UNC-SPERM Cryo-preserved spermatozoa $564.00 / Non-Profit Aliquot Approximate quantity3
    037520-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.