Strain Detail Sheet

Strain Name:
B6.Cg-Mapttm1(EGFP)Klt/Mmjax ES cell line ACB001
Stock Number:
037554-JAX
Citation ID:
RRID:MMRRC_037554-JAX
Other Names:
ACB001, B6.Cg-Mapttm1(EGFP)Klt Tg(PDGFB-APPSwInd)20Lms/Mmjax ACB001 mES cells

Gene Information

Gene Details

Mapttm1(EGFP)Klt
Name: microtubule-associated protein tau; targeted mutation 1, Kerry Lee Tucker
Synonyms: tauGFP, tau ko, Mapttm1(GFP)Klt
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Knockout
EGFP
Name: enhanced green fluorescent protein
Type: Gene
Species: Aequorea victoria (jellyfish)
Chromosome:
Alteration at locus: Knockout
Mapt
Name: microtubule-associated protein tau
Synonyms: Tau, Mtapt
Type: Gene
Species: Mouse
Chromosome: 11
Alteration at locus: Knockout
NCBI: 17762
HGNC: HGNC:6893
Homologene: 74962
Genetic Alterations
This ES cell line carries a Mapt knockout through an insertion of a cassette fusing EGFP and a neomycin resistance gene in Mapt exon 1.
Genotype Determination
Updated ES Cell information is included with the shipment
ES Cell Line
J1 derived from 129S4/SvJae

Strain Description

Strain of Origin
multiple
Strain Development

The ACB001 embryonic stem cell line was established using day 3.5 blastocysts from a cross between a hemizygous B6.Cg-Zbtb20Tg(PDGFB-APPSwInd)20Lms/Mmjax female (MMRRC:34836) and a C57BL/6-congenic male homozygous for Mapttm1(EGFP)Klt. This clone (ACB001) is one of two wild-type controls (see also MMRRC:37555) for a line carrying both alleles. The ACB001 line is heterozygous for Mapttm1(EGFP)Klt; it does not carry the Tg(PDGFB-APPSwInd)20Lms transgene.

These cell lines have been proven useful in in-vitro experiments directed towards the differentiation of ESCs to cortical neurons. Cell lines were derived and maintained on mitomycin-C treated mouse embryonic fibroblast feeder cells (MEFs) derived from C57BL/6J embryos.

Strain Origin

Donor
Laura Reinholdt, Ph.D., The Jackson Laboratory.
Primary Reference
Czechanski A, Byers C, Greenstein I, Schrode N, Donahue LR, Hadjantonakis AK, Reinholdt LG. Derivation and characterization of mouse embryonic stem cells from permissive and nonpermissive strains. Nat Protoc. 2014 Mar;9(3):559-74. (Medline PMID: 24504480)

Colony and Husbandry Information

Special Considerations

ES cells are expanded upon request and are usually ready for shipment in 3-5 weeks.

Health Status Report

Requested cell lines are tested by PCR analysis for mycoplasma spp. and Murine Parvoviruses (MPV) upon completion of expansion and prior to shipment.

Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email csmmrrc@jax.org.

Order Request Information

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
037554-JAX-CELL B6.Cg-Mapttm1(EGFP)Klt/Mmjax ES cell line ACB001 $546.00 / $546.00
Non-Profit / For-Profit		 Vial Includes genetic confirmation
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.