Strain Name:
STOCK Tg(hs692-cre/ERT2,-GFP)103Jlr/Mmucd
Stock Number:
037573-UCD
Citation ID:
RRID:MMRRC_037573-UCD
Other Names:
692-CreER-IRES-GFP
Major Collection:

Strain Information

GFP
Name: green fluorescent protein
Type: Gene
Species: Aequorea victoria (jellyfish)
Chromosome:
cre/ERT2
Name: cre tamoxifen-dependent recombinase
Type: Gene
Species: Multi-species
Chromosome:
Insc
Name: INSC spindle orientation adaptor protein
Synonyms: Inscuteable, 3830422K02Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233752
Homologene: 78662
SOX6
Name: SRY-box 6
Type: Gene
Species: Homo sapiens (human)
Chromosome: 11
Tg(hs692-cre/ERT2,-GFP)103Jlr
Name: transgene insertion 103, John Rubenstein
Synonyms: 692-CreER-IRES-GFP
Type: Transgene
Species: Mus musculus (mouse)
Chromosome:
Alteration at locus: Transgenic (cre)
Genetic Alterations
This mutant line harbors a randomly inserted transgene consisting of the human enhancer element hs692, which is flanked on human Chr 11 by the inscuteable homolog (Drosophila) (INSC) and SRY-box 6 (SOX6) genes, followed by a minimal Hsp68 promoter, the cre/ERT2 fusion gene, an internal ribosomal entry site (IRES), and a green fluorescent protein reporter gene. The fusion gene encodes Cre recombinase joined to a modified ligand-binding domain of the human estrogen receptor that preferentially binds tamoxifen or 4-hydroxytamoxifen, but not endogenous 17beta-estradiol; upon binding to tamoxifen, the cre/ERT2 protein is translocated to the nucleus.
Genotype Determination
ES Cell Line
NA
Phenotype

Homozygous: Not characterized

Hemizygous: No Phenotype

Cre-excised Phenotype: Undetermined

Strain Development
This mutant line harbors a randomly inserted transgene consisting of the human enhancer element hs692, which is flanked on human Chr 11 by the inscuteable homolog (Drosophila) (INSC) and SRY-box 6 (SOX6) genes, followed by a minimal Hsp68 promoter, the cre/ERT2 fusion gene, an internal ribosomal entry site (IRES), and a green fluorescent protein reporter gene. The fusion gene encodes Cre recombinase joined to a modified ligand-binding domain of the human estrogen receptor that preferentially binds tamoxifen or 4-hydroxytamoxifen, but not endogenous 17beta-estradiol; upon binding to tamoxifen, the cre/ERT2 protein is translocated to the nucleus.
Stable transgenic mice were generated by pronuclear injection using the FVB strain. Mice were later maintained on a C57BL/6J and CD1 background.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Developmental Biology
  • Neurobiology
Donor
Rubenstein [M.D.] [Ph.D.], John

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
White and Brown
MMRRC Breeding System
Backcross
Generation
5-6
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Undetermined Undetermined
Homozygotes are fertile: Undetermined Undetermined
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 8 months Greater than 12 months
Average litter size
5-9
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
037573-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
037573-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
037573-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.