Strain Name:
B6.129S4-Hrastm1Rak/Mmjax
Stock Number:
037589-JAX
Citation ID:
RRID:MMRRC_037589-JAX
Other Names:
HrasG12V, GV

Strain Information

Hrastm1Rak
Name: Harvey rat sarcoma virus oncogene; targeted mutation 1, Raju Kucherlapati
Synonyms: HarasG12V, GV
Type: Allele
Species: Mus musculus (mouse)
Alteration at locus: Conditional
Hras
Name: Harvey rat sarcoma virus oncogene
Synonyms: Ha-ras, Harvey-ras, H-ras, c-H-ras, Hras-1, c-rasHa, Hras1
Type: Gene
Species: Mouse
Chromosome: 7
Alteration at locus: Conditional
NCBI: 15461
HGNC: HGNC:5173
Homologene: 55890
Genetic Alterations
A G12V mutant form of protein is conditionally expressed from the Hras1 (Harvey rat sarcoma virus oncogene 1) gene when a neomycin cassette is excised by Cre recombinase. This oncogene is involved with the development of Costello syndrome, a neuro-cardio-facio-cutaneous developmental syndrome.
Genotype Determination
ES Cell Line
J1
Phenotype
These GV mutant mice conditionally express a mutant form of HRAS (Harvey rat sarcoma virus oncogene), which encodes a member of the Ras oncogene family involved in signal transduction pathways. Mutations in Hras are associated with Costello syndrome, a rare disorder associated with delayed development, craniofacial abnormalities, extra folds of skin, and multiple heart defects. The amino acid substitution, G12V, has the lowest GTPase activity found in codon 12 substitutions and is the predominant codon 12 mutation found in human Costello syndrome patients. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will express the mutant G12V protein in cre-expressing tissues. Mice heterozygous for the allele are viable and fertile.
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

  • normal phenotype
Strain Development
The targeting vector contains a loxP-flanked neomycin cassette upstream of a modified exon 2, which contains a G12V point mutation with a unique restriction site. The mutation results in a glycine to valine change at position 12. The construct was electroporated into J1 embryonic stem (ES) cells derived from 129S4/SvJae mice. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 females and offspring were backcrossed to C57BL/6 for at least 7 generations. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Cancer
  • Cardiovascular
  • Developmental Biology
  • Hematology
  • Model for Human Disease
    • Costello Syndrome
Donor
Kucherlapati [Ph.D.], Raju

Colony and Husbandry Information

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
Black
MMRRC Breeding System
Backcross
Generation
N7
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Undetermined Undetermined
Homozygotes are fertile: N/A N/A
Heterozygotes are fertile: N/A N/A
Age Reproductive Decline: Greater than 12 mont Greater than 12 mont
Average litter size
5-9
Recommended wean age
3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
037589-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
037589-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.