Strain Name:
STOCK Egfrtm1Rdk/Mmucd
Stock Number:
037646-UCD
Citation ID:
RRID:MMRRC_037646-UCD
Other Names:
EGF-R

Strain Information

Egfrtm1Rdk
Name: epidermal growth factor receptor; targeted mutation 1, Rik Derynck
Synonyms: EGF-R -
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Knockout
Egfr
Name: epidermal growth factor receptor
Synonyms: avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, Erbb, Wa5, 9030024J15Rik, Errb1
Type: Gene
Species: Mouse
Chromosome: 11
Alteration at locus: Knockout
NCBI: 13649
HGNC: HGNC:3236
Homologene: 74545
Genetic Alterations
A neomycin selection cassette was inserted into exon 2, which encodes the amino-terminal segment of the protein
Genotype Determination
ES Cell Line
JM-1 derived from 129X1/SvJ
Phenotype

Homozygous: mice survive for up to 8 days after birth and suffer from impaired epithelial development in several organs, including skin, lung, and gastrointestinal tract

Heterozygous: Normal weight and fertility

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

MeSH Terms
  • Animals
  • Female
  • Gene Targeting
  • Humans
  • Intestines/pathology
  • Lung/pathology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Multiple Organ Failure/genetics
  • Multiple Organ Failure/pathology
  • Receptor, Epidermal Growth Factor/deficiency
  • Receptor, Epidermal Growth Factor/genetics
  • Receptor, Epidermal Growth Factor/physiology
Strain Development
Animals bearing the targeted EGF-R allele were interbred and the F2 offspring were derived from matings between chimeras and C57BL/6J females. No difference was noted between their F2 offspring and the Swiss Webster Black F2 mice.
The targeting vector was introduced to JM-1 embryonic stem cells by electroporation. Correctly targeted ES cells were injected into C57BL/6J blastocysts. Chimeric males were mated with Swiss Webster Black females. Animals bearing the targetted EGF-R allele were interbred and the F2 offspring analyzed further by Southern blotting. On a few occasions, F1 animals were derived from matings between the chimaeras and C57BL/6J females, but no difference was noted between their F2 offspring and the Swiss Webster Black F2 mice.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Dermatology
  • Developmental Biology
  • Internal Organ
Donor
Zena Werb, Ph.D., University of California, San Francisco.
Rik Derynck, Ph.D., UCSF.
Primary Reference

Miettinen PJ, Berger JE, Meneses J, Phung Y, Pedersen RA, Werb Z, Derynck R. Epithelial immaturity and multiorgan failure in mice lacking epidermal growth factor receptor. Nature. 1995 Jul 27; 337-41. (Medline PMID: 7630400)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
Eye
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)

Wild-type female x Heterozygous male from the colony or reciprocal mating

Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 10 months 10 months
Average litter size
5-9
Recommended wean age
3-4 weeks
Average Pups Weaned
5-9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
037646-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.