Strain Name:
C57BL/6N-Klm1Anu/Mmucd
Stock Number:
041326-UCD
Citation ID:
RRID:MMRRC_041326-UCD
Other Names:
ENU26:008:Klotho, C57BL/6NCrlAnu-Klm1Anu/AnuApb

Strain Information

Klm1Anu
Name: klotho; mutation 1, Australian National University
Synonyms: ENU26:008:Klotho
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
Kl
Name: klotho
Synonyms: alpha-kl
Type: Gene
Species: Mouse
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 16591
HGNC: HGNC:6344
Homologene: 68415
Genetic Alterations
The mutation is an ENU-induced G to A change at nucleotide 1094 in Exon 2 resulting in an amino acid change from Tryptophan to STOP at position 328.
Phenotype
Homozygous: Macropathology: The mouse displayed moderate dehydration and respiratory distress before euthanasia. Loops of small intestine were noted in the thoracic cavity upon opening of the thoracic cavity indicating the presence of a diaphragmatic hernia. The spleen was very small and the kidneys were bronze. No masses were noted on the paws at necropsy. Histopathology: Brain, salivary gland, liver, oesophagus, ileum, caecum, colon, pancreas, lung unremarkable. Kidney: The kidney displays severe mineralisation of the cortex. The areas of mineralisation are multifocal to coalescing and involve the tubular tissues as well as blood vessel walls. There is golden pigment present in the medullary tubules and collecting ducts. Duodenum: The lamina propria of the duodenum is effaced by the presence of large amounts of mineralisation. Heart: The heart displays severe multifocal mineralisation of the aorta and large blood vessels. In addition, there is severe mineralisation of tracheal cartilage rings and submucosa (still attached to heart). Stomach: The glandular stomach displays severe dilatation of occasional gastric glands with necrotic cellular content as well as fragments of calcium. In addition, severe mineralisation of all small blood vessels and the entire submucosa of the glandular stomach is apparent. Furthermore, moderate mineralisation of the smooth muscle of the tunica muscularis is also evident. Spleen: The spleen indicates an immunocompromised phenotype with a complete absence of periarteriolar lymphoid sheaths. Minimal haemopoiesis is evident in the red pulp. Paws: There is evidence of small, focal areas of mineralisation in the dermal tissue, connective tissue as well as adjacent to the bone. The bones display moderate medullary haemorrhage and lack of trabeculae which may be related to excessive bone destruction resulting in hypercalcaemia. Diagnosis: Severe, chronic, multifocal mineralisation of glandular stomach, kidney, aorta, duodenum, trachea, dermis/connective tissue of paws. Minimal splenic haemopoiesis and absence of periarteriolar lymphoid sheaths.
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
Donor
Chris Goodnow, Ph.D., F.A.A., F.R.S., Australian National University.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
MMRRC Breeding System
Other or uncertain
Breeding Scheme(s)
  • Wild-type (C57BL/6) female x Heterozygous male or reciprocal mating
  • Wild-type female x Heterozygous male littermate or reciprocal mating
  • Heterozygous female x Heterozygous male littermate
Generation
G5
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Undetermined Undetermined
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
5
Recommended wean age
3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041326-UCD-RESUS Litter recovered from cryo-archive $5,055.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.