Strain Name:
B6.129P2-Syn1tm1Pggd/Mmjax
Stock Number:
041436-JAX
Citation ID:
RRID:MMRRC_041436-JAX
Other Names:
Synapsin I Knockout, Syn1 KO

Strain Information

Syn1tm1Pggd
Name: synapsin I; targeted mutation 1, Paul Greengard
Synonyms: Syn1tm1Lli, SynI-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Knockout
Syn1
Name: synapsin I
Synonyms: Syn-1, Synapsin Ib, Synapsin Ia
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Knockout
NCBI: 20964
Homologene: 48483
Genetic Alterations
Targeted deletion resulting in knockout of expression for Synapsin I. Homozygous synapsin I knockout mice exhibit seizures, delayed axonal outgrowth and delayed synapse formation. These mice may be useful in studying the role of synapsin I in neurodevelopment.
Genotype Determination
ES Cell Line
E14
Phenotype
Syn1, the most abundant member of the synapsin family of neuronal phophoproteins, is associated with neurotransmitter release and synaptogenesis. Synapsin I localizes to presynaptic terminals. Synapsin proteins are associated with autism. The synapsin I knockout allele has a PGK-neo cassette replacing exon 1 of the Syn1 gene. Homozygous mice exhibit a delay in the outgrowth of presynaptic neurites, axonal elongation and synapse formation in hippocampal neurons. Beginning at two months of age, mice experience spontaneous seizures. Homozygous mice are viable and fertile with no reported reproductive deficiency.
Mammalian Phenotype Terms
Allelic Composition: Syn1tm1Lli/Syn1tm1Lli (Genetic Background: B6.129P2-Syn1tm1Lli )

Allelic Composition: Syn1tm1Lli/Syn1tm1Lli (Genetic Background: involves: 129/Sv )

MeSH Terms
  • Animals
  • Axons/physiology
  • Axons/ultrastructure
  • Blotting, Western
  • Brain/physiology
  • Cells, Cultured
  • Cerebellum/physiology
  • Cerebral Cortex/physiology
  • Corpus Striatum/physiology
  • Embryo, Mammalian
  • Female
  • Genotype
  • Hippocampus/physiology
  • Immunohistochemistry
  • Male
  • Mice
  • Mice, Neurologic Mutants
  • Neurons/physiology
  • Organ Specificity
  • Reference Values
  • Sex Characteristics
  • Stem Cells
  • Synapses/physiology
  • Synapses/ultrastructure
  • Synapsins/deficiency
  • Synapsins/genetics
Strain Development
A targeting vector was designed by Dr. Paul Greengard (Rockefeller University) to replace exon 1 of the synapsin I gene (Syn1) on chromosome X with a PGK-neomycin cassette. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts, and chimeric males were bred with C57BL/6J females to establish the colony. The resulting mice were bred with C57BL/6 wildtype mice for at least ten generations prior to sending males to The Jackson Laboratory MMRRC in 2015.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Developmental Biology
  • Models for Human Disease
    • Epilepsy
    • Autism
  • Neurobiology
Donor
Paul Greengard, Ph.D., The Rockefeller University.
Primary Reference

Chin LS, Li L, Ferreira A, Kosik KS, Greengard P. Impairment of axonal development and of synaptogenesis in hippocampal neurons of synapsin I-deficient mice. Proc Natl Acad Sci U S A. 1995 Sep 26;92(20):9230-4. (Medline PMID: 7568107)

Colony and Husbandry Information

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
Black
MMRRC Breeding System
Backcross
Breeding Scheme(s)
  • Wild-type (C57BL/6) female x Hemizygous male or reciprocal mating

Generation
>10
Overall Breeding Performance
Good
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: 6 to 8 months 6 to 8 months
Average litter size
5-9
Recommended wean age
4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041436-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
041436-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.