Strain Name:
STOCK Tg(CAG-His3.3Rainbow)1Dean/Mmnc
Stock Number:
042288-UNC
Citation ID:
RRID:MMRRC_042288-UNC
Other Names:
HISRainbow, His-FLP9

Strain Information

H2b
Name: histocompatibility-2, MHC; b variant
Type: Allele
Species: Mus musculus (mouse)
Chromosome: UN
Alteration at locus: Transgenic (random, gene disruption)
Zp3
Name: zona pellucida glycoprotein 3
Synonyms: Zp-3
Type: Gene
Species: Mouse
Chromosome: 5
Alteration at locus: Transgenic (random, gene disruption)
NCBI: 22788
Homologene: 5178
Tg(CAG-His3.3Rainbow)1Dean
Name: transgene insertion 1, Jurrien Dean
Synonyms: HISRainbow
Type: Transgene
Species: Drosophila melanogaster (fruit fly)
Chromosome:
Alteration at locus: Transgenic (random, gene disruption)
Genetic Alterations
Hemizygous transgenic mice which can randomly express histone H3.3-EGFP, H3.3 R26K-ECFP, or H3.3 K27R-Cherry. Genetic mosaic strategy to study specific H3.3 amino acid residues,effect of the mutant isoforms -Three different fluorescent proteins were fused with H3.3 mutants, forming H3.3eGFP, H3.3R26KeCFP and H3.3K27RmCherry,
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • Phenotype

    Homozygous: normal

    Hetero/Hemizygous: normal

    Cre-excised Phenotype: undetermined 

    Mammalian Phenotype Terms
    Allelic Composition: (Genetic Background: )

    • immune system
      • decreased susceptibility to autoimmune diabetes [] [PMID:8350054]
    MeSH Terms
    • Animals
    • Apoptosis/genetics
    • Gene Deletion
    • Genetic Loci
    • Germ Cells/cytology
    • Germ Cells/metabolism
    • Male
    • Meiosis/genetics
    • Mice
    • Nuclear Proteins/metabolism
    • RNA Precursors/metabolism
    • RNA, Messenger/genetics
    • RNA, Messenger/metabolism
    • RNA, Small Interfering/metabolism
    • Spermatogenesis/genetics
    • Testis/cytology
    • Transcription Elongation, Genetic
    • Bone and Bones/cytology
    • Cell Lineage
    • Clone Cells
    • Ectoderm/cytology
    • Ectoderm/growth & development
    • Endothelium/cytology
    • Endothelium/growth & development
    • Extremities/growth & development
    • Female
    • Germ Layers/cytology
    • Hematopoietic Stem Cells/cytology
    • Mesoderm/cytology
    • Mesoderm/growth & development
    • Organ Specificity
    • Regeneration/physiology
    • Stem Cells/cytology
    • Tendons/cytology
    • Tendons/growth & development
    • Chromatin/chemistry
    • Chromatin Immunoprecipitation/methods
    • DNA/genetics
    • DNA/metabolism
    • DNA Methylation
    • Gene Expression Regulation
    • Genome
    • Histones/chemistry
    • Histones/genetics
    • Histones/metabolism
    • Humans
    • Microarray Analysis
    • Promoter Regions, Genetic
    • Protein Processing, Post-Translational
    • Recombinant Fusion Proteins/genetics
    • Recombinant Fusion Proteins/metabolism
    Strain Development
    To establish pCAG-H3.3eGFP transgenic mice, the H3.3eGFP fragment from H3.3-pEGFP-N1 (gift from Dr Philippe Collas; PMID:20375147) was subcloned into pCAGEN. To produce ZP3-H2BmCherry transgenic mice, the 1.6 kb ZP3 promoter (Svoboda et al., 2001) and H2B cDNA cloned from mouse ovary RNA were inserted into pmCherry-N1. The HISRainbow construct was generated by sequentially ligating lox 2272+lox N+lox P, polyA×3+lox 2272, H3.3-eGFP, polyA+lox N, H3.3R26K-eCFP, polyA+lox P, H3.3K27R-mCherry, polyA+frt (all with 5′ XbaI restriction sites and 3′ NheI or EcoRI restriction sites) into the pCAGEN plasmid (Addgene) (PMID:21866153).

    The strain was FLPed with B6;SJL-Tg(ACTFLPe)9205Dym/J (Jackson Strain #003800)
    Suggested Control Mice
    Wild-type littermates
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Cell Biology
    • Developmental Biology
    • Models for Human Disease
    • Reproduction
    • Research Tools
    Donor
    Dr. Jurrien Dean, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health
    Primary Reference

    Delbarre E, Jacobsen BM, Reiner AH, Sørensen AL, Küntziger T, Collas P. Chromatin environment of histone variant H3.3 revealed by quantitative imaging and genome-scale chromatin and DNA immunoprecipitation. Mol Biol Cell. 2010 Jun 1;21(11):1872-84. doi: 10.1091/mbc.E09-09-0839. Epub 2010 Apr 7. Erratum in: Mol Biol Cell. 2010 Nov;21(21):3759. (Medline PMID: 20375147)

    Rinkevich Y, Lindau P, Ueno H, Longaker MT, Weissman IL. Germ-layer and lineage-restricted stem/progenitors regenerate the mouse digit tip. Nature. 2011 Aug 24;476(7361):409-13. doi: 10.1038/nature10346. (Medline PMID: 21866153)

    Zhou L, Canagarajah B, Zhao Y, Baibakov B, Tokuhiro K, Maric D, Dean J. BTBD18 Regulates a Subset of piRNA-Generating Loci through Transcription Elongation in Mice. Dev Cell. 2017 Mar 13;40(5):453-466.e5. doi: 10.1016/j.devcel.2017.02.007. (Medline PMID: 28292424)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
    Coat Color
    Black or Brown
    Eye
    Red
    MMRRC Breeding System
    Backcross
    Generation
    N4 (C57BL/6), F2
    Overall Breeding Performance
    Good
    NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Reduced Reduced
    Homozygotes are fertile: Reduced Reduced
    Hetero/Hemizygotes are fertile: Reduced Reduced
    Age Reproductive Decline: 6 to 8 months 6 to 8 months
    Bred to Homozygosity
    No
    Average litter size
    4-6
    Recommended wean age
    3 weeks
    Average Pups Weaned
    4-6

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    042288-UNC-SPERM Cryo-preserved spermatozoa $564.00 / Non-Profit Aliquot Approximate quantity3
    042288-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.