Strain Detail Sheet

Strain Name:
STOCK Hand1tm4Abfi/Mmmh
Stock Number:
043548-MU
Citation ID:
RRID:MMRRC_043548-MU
Other Names:
Hand1stopfloxHand1T107;S109D, Hand1PO4+

Strain Information

Gene Details

Hand1tm4.1Abfi
Name: targeted mutation 4, A. Firulli (T107D/S109D)
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 4
Hand1
Name: heart and neural crest derivatives expressed 1
Synonyms: Ehand1, bHLHa27, Thing1, Th1, Hxt, eHAND
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15110
HGNC: HGNC:4807
Homologene: 3545
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Genetic Alterations

A floxed neo STOP cassette is inserted in the Hand1 5'UTR along with two point mutations: 

  • Hand1 (Gene ID 15110)
  • mRNA reference: NM_008213.2
  • protein reference: NP_032239.1
  • Targeted amino acid #1: p.Thr107Asp (T107D) via triple base change c.319A>G, c.320C>A, and c.321A>C
  • Targeted amino acid #2: p.Thr109Asp (S109D) via double base substitution c.325A>G and c.326G>A

Cre recombination removes the STOP cassette and allows for endogenous level of expression in tissues where Cre and Hand1 expression overlap.

Genotype Determination
ES Cell Line
CCE916 derived from 129S/SvEv

Strain Description

Phenotype

Homozygous: lethal E9.5

Hetero/Hemizygous: viable and fertile

Cre-excised Phenotype: Undetermined 

Strain Development
  • Based on submission information (and standard procedure assumptions) we determined the following:
  • Generation of targeting vector, which introduces a floxed neomycin stop cassette into the 5’UTR of Hand1 along with point mutations to produce p.Thr107Asp/p.Thr109Asp (this strain) p.Thr107Ala/p.Ser109Ala (MMRRC:43547)
  • Electroporation of CCE916 ES cells followed by G418 selection
  • Clonal expansion followed by Southern blot RFLP analysis (or PCR) to confirm recombinant clones.  
  • Injection of desired clones into C57BL/6 blastocysts to produce chimeras.
  • Mating of chimera with C57BL/6
  • Maintenance on mixed C57BL/6, 129/SvJ, NIH Swiss background
Suggested Control Mice
Wild-type littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Research Applications

  • Cardiovascular
  • Developmental Biology

Strain Origin

Donor
Anthony Firulli, Ph.D., Indiana University School of Medicine.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
black agouti white

Breeding

MMRRC Breeding System
Sib-mating
Breeding Scheme(s)
male het with female gets
Generation
sib mating
Overall Breeding Performance
Excellent

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 8 to 12 months 8 to 12 months
Bred to Homozygosity
No
Average litter size
5-9
Recommended wean age
4 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.