Strain Detail Sheet

Strain Name:
FVB-Ascl1em1Jesw/Mmucd
Stock Number:
043552-UCD
Citation ID:
RRID:MMRRC_043552-UCD
Other Names:
Ascl1-TdTomato2, M2A, Mash1-TdTomato2

Strain Information

Gene Details

Ascl1em1Jesw
Name: achaete-scute family bHLH transcription factor 1; endonuclease-mediated mutation 1, James Schwob
Synonyms: Mash1-TdTomato2, Ascl1-TdTomato2, M2A
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Knock-In
Ascl1
Name: achaete-scute family bHLH transcription factor 1
Synonyms: ASH1, Mash1, bHLHa46
Type: Gene
Species: Mouse
Chromosome: 10
Alteration at locus: Knock-In
NCBI: 17172
HGNC: HGNC:738
Homologene: 31234
Genome Browser [Click image to go to Jbrowse]



Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
This is a CRISPR mediated knock-in, replacing endogenous Ascl1 (Gene ID:17172) with the same gene that has been altered to add two TdTomato (GenBank:AY678269) open reading frames separated by self-cleaving peptide 2A sequences (i.e., Ascl1-2A-TdTomato-2A-TdTomato), enabling expression of individual proteins, TdTomato and Ascl1.
Genotype Determination
Genotyping protocol

Strain Description

Phenotype

Homozygous: TdTomato fluorescence in Ascl1 expressing cells. No effect on viability or fertility.

Hetero/Hemizygous: TdTomato fluorescence in Ascl1 expressing cells. No effect on viability or fertility.

Cre-excised Phenotype: Undetermined.

Strain Development
Superovulated FVB females were harvested for embryos, and Cas9 mRNA, gRNA, and linear donor plasmid were microinjected. Embryos were transplanted to Swiss Webster foster females. Line has been maintained and backcrossed on the FVB background for 2 generations. The line is kept as heterozygotes, although for no particular reason.
Suggested Control Mice
Wild-type littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Research Applications

  • Cell Biology
  • Developmental Biology
  • Neurobiology
  • Research Tools

Strain Origin

Donor
James Schwob, M.D., Ph.D., Tufts University, Sackler School.

Colony and Husbandry Information

Special Considerations

No special care necessary

Health Status Report

Colony Surveillance Program and Current Health Reports

For more information about this colony's health status contact mmrrc@ucdavis.edu

Appearance

Coat Color
White

Breeding

MMRRC Breeding System
Backcross
Generation
2
Overall Breeding Performance
Excellent

Reproductive Statistics

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Undetermined Undetermined
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Bred to Homozygosity
No
Average litter size
5-9
Recommended wean age
3-4 weeks

Order Request Information

Availability Level

When this strain becomes available, Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.