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Availability & Fees Order this Strain
Genotyping Protocol
Homozygous: None, unless bred to Cre strain.
Hetero/Hemizygous: None noted
Cre-excised Phenotype:
Embryonic: Tie2-Cre; Pak2fl/fl embryos: At E9.5, all of the ePak2 KO embryos showed various levels of growth and developmental retardation and defective vascularization. The embryos with the most severe phenotypes had not turned and displayed an open neural tube and appeared degraded (Fig. 1C, right panel, and F, bottom right panel). ePak2 KO embryos displayed various levels of pericardial edema which is indicative of cardiac dysfunction. The yolk sacs from ePak2 KO embryos were pale, and the prominent vitelline vessel and smaller branched vessels were absent.
Adult: ePak2 KO sagittal sections showed focal areas of cleaved caspase 3 in embryos with a moderate phenotype and more widespread cleaved caspase 3 immunostaining in embryos with a severe phenotype.
Dorrance, A.M., De Vita, S., Radu, M., Reddy, P.N.G., McGuinness, M.K., Harris, C.E., Mathieu, R., Lane, S.W., Kosoff, R., Milsom, M.D., Chernoff, J.,* and Williams, D.A. * The Rac GTPase effector p21 Activated Kinase-2 is essential for hematopoietic stem/progenitor cell migration and engraftment. Blood 121:2474-82, 2013 *(co-senior authors) (Medline PMID: 23335370)
Radu, M. and Chernoff, J. An in vivo assay to test blood vessel permeability. J. Vis. Exp. 16:e50062, 2013. (Medline PMID: 23524912)
Phee, H., Au-Yeung, B.B., Pryshchep, O., O'Hagan, K.L., Fairbairn, S.G., Radu, M., Kosoff, R., Mollenauer, M., Cheng, D., Chernoff, J., and Weiss, A. Pak2 is required for actin cytoskeleton remodeling, TCR signaling, and normal thymocyte development and maturation. Elife 3:e02270, 2014. (Medline PMID: 24843022)
Zeng, Y., Broxmeyer, H.E., Staser, K., Chitteti, B.R., Park, S.J., Hahn, S., Cooper, S., Sun, Z., Jiang, L., Yang, X., Yuan, J., Kosoff, R., Sandusky, G., Srour E.F., Chernoff J., and Clapp D.W. Pak2 regulates hematopoietic progenitor cell proliferation, survival and differentiation. Stem Cells 33:1630-41, 2015. (Medline PMID: 25586960)
Kosoff,, R.E., Aslan, J.E., Kostyak, J.C., Dulaimi, E., Chow, H.-Y., Prudnikova, T.Y., Radu, M., Kunapuli, S.P., McCarty, O.J.T., and Chernoff, J. Pak2 restrains endomitosis during megakaryopoiesis. Blood 125:2995-3005, 2015. (Medline PMID: 25824689)
Pak2 Links TCR Signaling Strength to the Development of Regulatory T Cells and Maintains Peripheral Tolerance, O'Hagan, K.L., Choi, J., Pryshchep, O., Chernoff, J., and Phee, H. J. Immunol. 195:1564-1577, 2015. (Medline PMID: 26157175)
Radu, M., Lyle, K., Hoeflich, K.P., Villamar-Cruz, O., Koeppen, H., and Chernoff, J. p21-activated kinase 2 (Pak2) regulates endothelial development and function through Bmk1/Erk5 pathway. Mol. Cell. Biol. 35:3990-2005, 2015 (Medline PMID: 26391956)
Reddy, P.N.G., Radu, M., Xu, K., Wood, J., Harris, C.E., Chernoff, J., and Williams, D.A. p21 activated kinase 2 regulates HSC cytoskeleton, migration and homing via CDC42 activation and interaction with b-Pix. Blood, 127:1967-1975, 2016. (Medline PMID: 26932803)
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.