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Availability & Fees Order this Strain
Homozygous: B cells from these mice fail to class switch so mice do not develop IgG responses
Hetero/Hemizygous: Immunization with NP-CGG (nitrophenol conjugated to chicken gamma globulin) yielded anNP-specific IgM antibody titer that was slightly higher in sera of AID-/- chimera than those of AID+/- chimera. However, no anti-NP IgG1 was produced by AID-/- chimera. MLN RNA (VH186.2-NP response) from AID+/- and AID-/- chimeras showed that AID+/- derived VH186.2 sequences of the mu and gamma1 chains carried comparable levels of mutation in the total and framework regions. However, CDR of the AID+/- gamma1 chain contained a 60% higher frequency of mutations than that of the AID+/- mu chain. R/S ratios were higher in CDR than framework region of both mu and gamma1 chains of AID+/- VH186.2 sequences. Furthermore, AID+/- VH186.2 sequences of the gamma1 chain contained, more frequently than those of the mu chain, the characteristic mutation at 33Trp (TGG) to 33Leu (TTG), which is known to be responsible for high-affinity binding to the NP hapten By contrast, AID-/- mu chains contained 10 times fewer mutations than AID+/- mu chain. There were no mutations in CDR 1 and CDR 2 of the AID-/- VH186.2 mu chain. The mutation frequency (1.5 x 10e-3) of AID-/- VH186.2 sequences was comparable to that due to PCR by Taq.
Cre-excised Phenotype:
In this strain, activation-induced cytidine deaminase (Aicda - Gene ID 116298) was disrupted. To that end TT2 embryonic stem cells (strain of origin: (C57BL/6NCrlj x CBA/JNCrlj)F1) were electroporated with a target vector (see Fig. 2A in PMID:11007474) designed to replace exon 2 and the 5’ half of exon 3 with the neomycin resistance (NeoR) gene. Homologous integration was confirmed by PCR screening and Southern blot hybridization. ES cells were injected into ICR 8-cell embryos and implanted into pseudopregnant females. The resulting founder chimeras were crossed into C57BL/6 background to generate C57BL/6-Aicda‑/-mice, which were further crossed to MRL/MpJ-Faslpr/J(“MRL/lpr” - JAX Stock #000485) for 12 generations, then intercrossed to generate final strain. To prevent MRL/lpr drift, the animals were backcrossed to JAX Stock #000485 every 2 years.
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.