Strain Name:
C57BL/6J-Tg(Fcer1g-cre)NIDA168Htz/Mmucd
Stock Number:
043798-UCD
Citation ID:
RRID:MMRRC_043798-UCD
Other Names:
Fcer1g-Cre (NIDA168),

Strain Information

cre
Name: cre recombinase
Type: Gene
Species: Enterobacteria phage P1 (bacteriophage P1)
Chromosome:
Fcer1g
Name: Fc receptor, IgE, high affinity I, gamma polypeptide
Synonyms: FcR-gamma, FcRgamma, Fce1g, FcepsilonRI, Ly-50, FcR[g]
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14127
HGNC: HGNC:3611
Homologene: 3029
Genetic Alterations
A bacterial artificial chromosome (BAC; address RP23-395H6) was modified to hold a Cre recombinase transgene under the control of the Fcer1g promoter. The Cre recombinase possessed a poly-A tail to prevent expression of the Fcer1g gene from the BAC.
Genotype Determination
Phenotype

Homozygous: We do not recommend that the Fcer1g-Cre (NIDA168) mice be bred to homozygosity

Hetero/Hemizygous: If a Fcer1g-Cre (NIDA168) mouse is crossed with a mouse possessing a floxed allele, recombination is expected to occur in microglia.

Cre-excised Phenotype:

Strain Development
The RP23-395H6 bacterial artificial chromosome was modified as described in Gong et al (2010) Nat Protoc 5:1678-1696. The background of the embryos that were injected was C57BL/6J. All subsequent generations were maintained on this background. The mice were obtained from Jackson. The catalog number was Jackson 000664

See image here.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
Donor
Nathaniel Heintz, Ph.D., The Rockefeller University.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
The MMRRC at UC Davis performed microbita analysis on feces samples received from the donating investigator while the mice were still at their facility. There is a MMRRC Fecal Microbiota report available for this strain.If you are interested in having the MMRRC at UC Davis perform microbiota analysis once the line is established at your facility please contact mmrrc@ucdavis.edu.
Coat Color
Black
Other
Attached is a sagittal section through the brain of a Fcer1g-Cre, NIDA168 mouse (x Rosa26-LSL-EYFP) processed for anti-EGFP DAB immunohistochemistry. There is clear recombination in microglia scattered throughout the brain
MMRRC Breeding System
Other or uncertain
Breeding Scheme(s)
We recommend that hemizygous Fcer1g-Cre (NIDA168) mice be bred with wild type C57BL/6J mice. The resulting litters will be 50% transgenic and 50% wild type.
Generation
N/A
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Undetermined Undetermined
Homozygotes are fertile: Undetermined Undetermined
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 8 to 12 months 8 to 12 months
Bred to Homozygosity
No
Average litter size
4-6
Recommended wean age
3-4 weeks
Average Pups Weaned
4-6

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043798-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
043798-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
043798-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.