Strain Name:
B6.129S6-Acer3tm1Cmao/Mmjax
Stock Number:
043827-JAX
Citation ID:
RRID:MMRRC_043827-JAX
Other Names:
ACer 3-/-

Strain Information

Acer3tm1Cmao
Name: alkaline ceramidase 3; targeted mutation 1, Cungui Mao
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Knockout
Acer3
Name: alkaline ceramidase 3
Synonyms: 5430429L08Rik, Phca, 1110057L18Rik
Type: Gene
Species: Mouse
Chromosome: 7
Alteration at locus: Knockout
NCBI: 66190
Homologene: 5410
Genetic Alterations
An Acer3-targeting vector was constructed so that upon homologous recombination between the targeting vector and the WT Acer3 allele, exon 8 of the Acer3 gene was replaced by the neomycin resistant gene (Neo) cassette. Knock-out mice exhibit cerebellar ataxia, Purkinje cell degeneration and an exacerbated response to DSS-induced colitis and colitis-associated tumorigenesis. This strain may be useful for studying the role of ceramidase in sphingolipid homeostasis in the brain and inflammatory diseases.

ES Cell Line
TC1/TC-1
Phenotype
Acer3 (alkaline ceramidase 3) encodes a ceramidase that catalyzes the hydrolysis of saturated long-chain ceramides into sphingosine. ACER3 is the most abundant of the alkaline ceramidase family and is upregulated in the mouse brain with aging. The Acer3- allele contains a neomycin cassette used to replace exon 8. Catalytic activity of ACER3 is markedly decreased, but not completely abolished in these mice. Mice homozygous for the mutation are viable and fertile. Loss of ACER3 results in an increase in the total levels of ceramides in the cerebellum (and to a lesser extent in the cerebrum), a decrease in both sphingosine and phosphorylated sphingosine (S1P) by 8 months of age in both the cerebrum and cerebellum and Purkinje cell degeneration. Beginning at 8 months of age, rearing activity is decreased, latency to fall (as assessed by rotarod) is increased, beam walking is impaired, and hindlimb clasping is exhibited. In addition, ACER3 deficiency promotes LPS-induced expression of pro-inflammatory cytokines and aggravates DSS-induced colitis and colitis-associated tumorigenesis. This strain may be useful for studying the role of ceramidase in sphingolipid homeostasis in the brain and inflammatory diseases.
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

Strain Development
A targeting vector containing a neomycin resistance (neo) cassette was used to replace exon 8 of the Acer3 gene. The construct was electroporated into 129S6/SvEvTac-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to C57BL/6J mice and backcrossed for at 16 generations to establish a colony. Analysis by RT-PCR reveals that a truncated coding sequence is transcribed. Catalytic activity of ACER3 is markedly decreased, but not completely abolished. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice for at least one generation.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Cancer
  • Models for Human Disease
  • Neurobiology
Donor
Cungui Mao, Ph.D., Stony Brook University.

Colony and Husbandry Information

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
Black
MMRRC Breeding System
Sib-mating
Breeding Scheme(s)
Sibling mating with a cross to C57BL/6J mice from Jackson Laboratories once every three years.
Generation
N16F?
Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Less than 4 months Less than 4 months
Bred to Homozygosity
Yes
Average litter size
5-9
Recommended wean age
3 weeks
Average Pups Weaned
5-9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043827-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
043827-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.