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Chemically-induced point mutation in the Apc gene (MGI:88039) causing a truncation in the Apc protein.
HGVS nomenclature:
Homozygous: lethal
Hetero/Hemizygous: intestinal neoplasia, adenomas develop primarily in the small intestine with the preponderance of the adenomas developing in the distal half. MMRRC:43849 and MMRRC:50543 are genetically identical but harbor two distinct complex microbiota that alter the intestinal adenoma phenotype. The original ApcMin mice from the closed genetic colony from William Dove at the University of Wisconsin was rederived using surrogate dams that harbored two distinct microbiota populations: low complexity and high complexity. The original closed colony line is available through the MMRRC with either the low complexity (MMRRC:43849) or high complexity (MMRRC:50543) microbiota that have the represented phenotypes in the small intestine and colon.
Su LK, Kinzler KW, Vogelstein B, Preisinger AC, Moser AR, Luongo C, Gould KA, Dove WF. Multiple intestinal neoplasia caused by a mutation in the murine homologof the APC gene. Science. 1992 May 1;256(5057):668-70. Erratum in: Science 1992May 22;256(5060):1114. (Medline PMID: 1350108)
Hart ML, Ericsson AC, Lloyd KCK, Grimsrud KN, Rogala AR, Godfrey VL, NielsenJN, Franklin CL. Development of outbred CD1 mouse colonies with distinctstandardized gut microbiota profiles for use in complex microbiota targetedstudies. Sci Rep. 2018 Jul 4;8(1):10107. doi: 10.1038/s41598-018-28448-0. (Medline PMID: 29973630)
MMRRC:43849 and MMRRC:50543 are genetically identical but harbor two distinct complex microbiota that alter the intestinal adenoma phenotype. The original ApcMin mice from the closed genetic colony from William Dove at the University of Wisconsin was rederived using surrogate dams that harbored two distinct microbiota populations: low complexity and high complexity (PMID:29973630). The original closed colony line is available through the MMRRC with either the low complexity (similar to JAX microbiota) or high complexity (similar to Envigo) microbiota that have the represented phenotypes in the small intestine (A) and colon (B).
Statistical testing demonstrates that both MMRRC:43849 and MMRRC:50543 develop significantly more tumors than ApcMinmice from the Jackson Laboratory (Strain #002020). MMRRC:50543 (high complexity microbiota) develop significantly more tumors than MMRRC:43849 (low complexity microbiota). While MMRRC:50543 has similar tumor numbers to ApcMinmice from McCardle Laboratory for Cancer Research, MMRRC:43849 has significantly fewer tumors than ApcMin from McCardle.
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.
A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Chase Bunger.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Chase Bunger
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.