Strain Detail Sheet

Strain Name:
C57BL/6J-Hcfc2sca/Mmmh
Stock Number:
043851-MU
Citation ID:
RRID:MMRRC_043851-MU
Other Names:
Hcfc2^sca, scaffold
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Hcfc2sca
Name: host cell factor C2; scaffold
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
Hcfc2
Name: host cell factor C2
Synonyms: 1700129L13Rik, fkls
Type: Gene
Species: Mouse
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 67933
Homologene: 8337
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Additional Resources
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GeneCards
ClinGen
Genetic Alterations
ENU mutagenesis induced a C-to-A point mutation (NM_001081218.1: c.1795T>A) that results in the amino acid substitution of lysine for threonine at position 599 (p.Phe599Ile or F599I).
Genotype Determination

Strain Description

Phenotype

Homozygous: Not Characterized

Hetero/Hemizygous: Decreased TNF secretion from macrophages of mice stimulated with polyinosinic:polycytidylic acid ("poly(I:C)").

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )
Strain Development
Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.
Suggested Control Mice
Wild-type littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Research Applications

  • Immunology and Inflammation

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

For more information about this colony's health status contact mmrrc@missouri.edu

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Generation
Unknown
Overall Breeding Performance
Excellent

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Bred to Homozygosity
No
Average litter size
4-6
Recommended wean age
3-4 weeks
Average Pups Weaned
4-6

Order Request Information

Availability Level

When this strain becomes available, Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.