Strain Detail Sheet

Strain Name:
C57BL/6-Rgs21tm1.1Dski/Mmnc
Stock Number:
046280-UNC
Citation ID:
RRID:MMRRC_046280-UNC
Other Names:

C57BL/6J-Rgs21tm1.1Dski/Mmnc,Rgs21 conditional knockout (flox'd exon 5)

Strain Information

Gene Details

Rgs21tm1.1Dski
Name: regulator of G-protein signalling 21; targeted mutation 1.1, David Siderovski
Synonyms: Rgs21fl
Type: Allele
Species: Mus musculus (mouse)
Chromosome:
Alteration at locus: Conditional
Rgs21
Name: regulator of G-protein signalling 21
Synonyms: EG624910
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Conditional
NCBI: 624910
Homologene: 82452
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Genetic Alterations
Conditional knockout of the Rgs21 gene by floxing exon 5. Involved insertion of loxP sites on either side of Rgs21 exon 5 (which encodes the final 67 amino-acids of the 152-amino acid RGS21 open-reading frame).
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    C57BL/6 ES Cell Line

    Strain Description

    Phenotype
    Rgs21-null mice have blunted aversion to quinine and denatonium, and blunted preference for monosodium glutamate, the sweeteners sucrose and SC45647, and (surprisingly) NaCl.

    Taste perception phenotypes are only elaborated when Cre-recombinase is cross-bred and expressed (or induced).
    Strain Development
    Conditional knockout of the Rgs21 gene was produced directly in the C57BL/6 background by genOway (Lyon, France). It involved insertion of loxP sites on either side of Rgs21 exon 5 (which encodes the final 67 amino-acids of the 152-amino acid RGS21 open-reading frame), as well as a neomycin-resistance gene (neo) flanked by “flippase recognition target” (FRT) sites, by homologous recombination in C57BL/6 embryonic stem cells. Drug-selected embryonic stem cells, with PCR and Southern blot evidence of homologous recombination, were subsequently injected into C57BL/6 blastocysts. Resulting chimeric male mice were crossed with females from the genOway proprietary ubiquitous Flp recombinase expressing mice for in vivo removal of the FRT-flanked neo selection cassette. F1 heterozygous Rgs21fl/+ mice devoid of the neo selection cassette were delivered by genOway to the Siderovski lab at WVU. This conditional ‘floxed’ Rgs21 knockout strain was maintained at WVU by backcrossing to the C57BL/6J strain.
    Suggested Control Mice
    C57BL/6J

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    Genetic QC analysis (GQC) was performed on representative samples from the donor submission and provides a baseline reference. Regardless of the material ordered (live mice, sperm, embryos, resuscitated animals), the provided material is guaranteed to contain the primary genetic alteration of interest; however, the composition of the genetic background may vary (See Reproducibility sections of the report for details). The impact of genetic background on previously reported phenotypes has not been evaluated.

    GQC analysis was done on two XY samples, WA9288 and JK5682, at the time of importation and the conclusions are consistent in all samples. The results of the analysis are congruent with the SDS and WA9288 is the representative sample selected for the report. The primary (host) background is C57BL/6 and there is positive evidence of the C57BL/6J and C57BL/6N substrains. The mitochondrial genome is consistent with the C57BL/6 background and the Y chromosome is consistent with at least one backcross to C57BL/6J. Both samples are homozygous for the C57BL/6N background at the locus. The following constructs are detected in all samples: IRES, r_FP?.

    The genome of MMRRC:046280 mice is 87% inbred at the substrain level. 13% of the genome is segregating for the two substrains,C57BL/6J and C57BL/6N. The contribution of C57BL/6J is 86.3%. The contribution of C57BL/6N is 13.7%.

    The estimated inbreeding, congenicity, and the relative strain/substrain contribution depend on the number of samples genotyped. The genomes of strains that are not fully inbred are not completely reproducible.

    ATTENTION: MiniMUGA has detected a potential unexpected construct (r_FP) that may interfere with the experimental design/rigor and reproducibility. It is recommended to genotype to ensure that r_FP is not present.

    Based on this report, a request has been made to update the strain name from C57BL/6J-Rgs21tm1.1Dski/Mmnc to C57BL/6-Rgs21tm1.1Dski/Mmnc
    MiniMUGA Sample Report
    MiniMUGA Sample Report Link

    Research Applications

    • Neurobiology

    Strain Origin

    Donor
    David Siderovski, Ph.D West Virginia University
    Primary Reference

    Schroer AB, Gross JD, Kaski SW, Wix K, Siderovski DP, Vandenbeuch A, Setola V.Development of Full Sweet, Umami, and Bitter Taste Responsiveness RequiresRegulator of G protein Signaling-21 (RGS21). Chem Senses. 2018 May23;43(5):367-378. doi: 10.1093/chemse/bjy024. (Medline PMID: 29701767)

    Colony and Husbandry Information

    Special Considerations

    N/A

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Appearance

    Coat Color
    Black
    Eye
    Black

    Breeding

    MMRRC Breeding System
    Random intra-strain mating
    Generation
    10
    Overall Breeding Performance
    Excellent

    Reproductive Statistics

    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: 8 to 12 months 8 to 12 months
    Average litter size
    7-9
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    5-9

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Conditions of Distribution

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    046280-UNC-SPERM Cryo-preserved spermatozoa $564.00 / Non-Profit Aliquot Approximate quantity3
    046280-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.