Strain Name:
C57BL/6N-Brd2em1(IMPC)Wtsi/Mmucd
Stock Number:
046824-UCD
Citation ID:
RRID:MMRRC_046824-UCD
Other Names:
CRISPR_DAAE,
Major Collection:

Strain Information

Brd2em1(IMPC)Wtsi
Name: bromodomain containing 2; endonuclease-mediated mutation 1, Wellcome Trust Sanger Institute
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: CRISPR
Brd2
Name: bromodomain containing 2
Synonyms: Ring3, Frg-1, D17H6S113E, Fsrg1, Rnf3
Type: Gene
Species: Mouse
Chromosome: 17
Alteration at locus: CRISPR
NCBI: 14312
HGNC: HGNC:1103
Homologene: 74540
Genetic Alterations
This is an Exon or Partial Exon deletion from the Brd2 (DAAE) gene using CRISPR Cas9 gene editing technology in mouse zygotes. Please refer to the genotyping protocol for further details on the deleted sequence and flanking region sequences.
Genotype Determination
Phenotype
Phenotyping data may be available at mousephenotype.org.
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

  • cardiovascular system
    • persistent right dorsal aorta []
    • persistent trigeminal artery []
    • retroesophageal right subclavian artery []
    • interrupted aortic arch, type b []
    • aorta coarctation []
    • abnormal common carotid artery morphology []
    • abnormal external carotid artery origin []
    • abnormal vertebral artery topology []
    • artery stenosis []
    • abnormal brain artery topology []
    • enlarged liver sinusoidal spaces []
    • abnormal umbilical vein topology []
    • abnormal ductus venosus morphology []
    • abnormal ductus venosus topology []
    • duplication of ductus venosus []
    • abnormal hepatic portal vein connection []
    • dual inferior vena cava []
    • abnormal pectinate muscle morphology []
    • absent pectinate muscle []
    • thin myocardium compact layer []
    • common truncal valve []
    • persistent truncus arteriosis []
    • double outlet right ventricle []
    • transposition of great arteries []
    • abnormal interatrial septum morphology []
    • atrioventricular septal defect []
    • perimembraneous ventricular septal defect []
    • muscular ventricular septal defect []
    • enlarged heart []
    • abnormal semilunar valve morphology []
    • abnormal aortic valve morphology []
    • abnormal pulmonary valve morphology []
    • hemorrhage []
  • craniofacial
    • basisphenoid bone foramen []
    • abnormal middle ear ossicle morphology []
    • abnormal nasal septum morphology []
  • embryo
    • abnormal umbilical vein topology []
    • abnormal Mullerian duct topology []
    • myelocele []
    • abnormal physiological umbilical hernia morphology []
  • endocrine/exocrine glands
    • abnormal adenohypophysis morphology []
    • abnormal pineal gland morphology []
    • small thymus []
    • abnormal thymus topology []
    • small thyroid gland []
    • abnormal gallbladder morphology []
  • growth/size/body
    • abnormal nasal septum morphology []
    • thoracoschisis []
    • fetal growth retardation []
    • heterochrony []
  • hearing/vestibular/ear
    • abnormal middle ear ossicle morphology []
  • hematopoietic system
    • small thymus []
    • abnormal thymus topology []
  • homeostasis/metabolism
    • retropleural edema []
    • subcutaneous edema []
  • immune system
    • small thymus []
    • abnormal thymus topology []
    • absent connection between subcutaneous lymph vessels and lymph sac []
    • blood in lymph vessels []
  • liver/biliary system
    • enlarged liver sinusoidal spaces []
    • abnormal gallbladder morphology []
  • muscle
    • abnormal pectinate muscle morphology []
    • absent pectinate muscle []
    • thin myocardium compact layer []
    • absent eye muscles []
  • nervous system
    • abnormal brain artery topology []
    • myelocele []
    • absent superior cervical ganglion []
    • reduced sympathetic cervical ganglion size []
    • exencephaly []
    • enlarged brain ventricles []
    • abnormal choroid plexus morphology []
    • abnormal forebrain morphology []
    • abnormal adenohypophysis morphology []
    • abnormal pineal gland morphology []
    • abnormal vagus ganglion morphology []
    • abnormal hypoglossal nerve topology []
    • abnormal olfactory nerve morphology []
    • abnormal optic chiasm morphology []
    • absent vagus nerve []
  • renal/urinary system
    • small kidney []
  • respiratory system
    • abnormal nasal septum morphology []
    • abnormal lung position or orientation []
  • skeleton
    • basisphenoid bone foramen []
    • abnormal middle ear ossicle morphology []
    • absent costovertebral joint []
    • decreased rib number []
    • increased rib number []
    • rib bifurcation []
    • rib fusion []
    • scoliosis []
    • abnormal vertebral arch morphology []
    • absent vertebral arch []
    • fusion of vertebral arches []
    • abnormal vertebral body morphology []
  • vision/eye
    • absent eye muscles []
    • abnormal optic chiasm morphology []
    • aphakia []
    • abnormal optic cup morphology []
    • abnormal optic stalk morphology []
Strain Development
CRISPR guide(s) and Cas9 protein were microinjected or electroporated into C57BL/6N zygotes and progeny were screened for the desired mutation. Founders were mated to C57BL/6N breeders, and derived N1 progeny were identified by PCR and/or sequencing. N1 were then mated again to C57BL/6N breeders, to generate N2 mice, identified by PCR and/or sequencing. In most cases, N2 heterozygous mutant mice were mated for production of phenotyping cohorts, and N2 or N2F1 heterozygous mice were used for cryopreservation purposes. The MMRRC will use C57BL/6N females for cryo-recovery.
Suggested Control Mice
C57BL/6N or wild-type from colony
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
Donor
The KOMP Repository, University of California, Davis; Originating from Ramiro Ramirez-Solis, Wellcome Sanger Institute

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
black
MMRRC Breeding System
Backcross or Sib-Mating

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
046824-UCD-RESUS Litter recovered from cryo-archive $5,055.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.