Strain Name:
FVB/NJ-Platr14em1Mbp/Mmucd
Stock Number:
050505-UCD
Citation ID:
RRID:MMRRC_050505-UCD
Other Names:
FVB/NCrl-Platr14, CR10063

Strain Information

Platr14em1Mbp
Name: pluripotency associated transcript 14; endonuclease-mediated mutation 1, Mouse Biology Program
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Platr14
Name: pluripotency associated transcript 14
Synonyms: 4930500J02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74959
Genetic Alterations
Knockin via CRISPR of IRES-EGFP-SV40 polyA cassette via CRISPR-Cas9 genome targeting of long non-coding RNA (lncRNA) of the Platr14 gene. This is for in vivo gene expression tracing during mouse embryonic development and generates a knockout (trap) of the lnc.
Genotype Determination
Phenotype
It was found to be homozygous lethal when breeding up to produce cohorts for phenotyping. Phenotyping will be performed on heterozygous mice only. Stage of lethality is unknown. It was also confirmed that embryos produced fluorescence at day E4.5.
Strain Development
An IRES-EGFP-SV40polyA cassette was injected into FVB/NCrl oocytes using CRISPR Cas9 endonucleases. Founder mice were screened and confirmed to contain the EGFP, and insertion into the selected region via PCR plus sequencing. These were bred to FVB/NCrl for two generations, and then intercrossed het x het at N2 for production of cohorts. No homozygous mice were derived from the het x het matings.
Suggested Control Mice
FVB/NCrl
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Developmental Biology
Donor

Ashley Benham, Ph.D Texas Medical Center, Texas Heart Institute, Joshua Wood, University of California Davis Mouse Biology Program.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
White
Eye
Pink
Other
This mouse model is being phenotyped in the comprehensive phenotyping pipeline as part of the ENCODE project.
MMRRC Breeding System
Sib-mating
Generation
N2F1
Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: N/A N/A
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
4-6
Recommended wean age
3 Weeks
Average Pups Weaned
4-6

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
050505-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
050505-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.