Strain Name:
Crl:CD1-Neurog2tm5(Neurog2)Fgu/Mmucd
Stock Number:
050509-UCD
Citation ID:
RRID:MMRRC_050509-UCD
Other Names:
Neurog2tm5(Neurog2)Fgu, Ngn2KIfloxNgn2flox, Ngn2KIloxNgn2

Strain Information

Neurog2tm5(Neurog2)Fgu
Name: neurogenin 2; targeted mutation 5, Francois Guillemot
Synonyms: Ngn2KIfloxNgn2flox, Ngn2KIloxNgn2
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Conditional
Neurog2
Name: neurogenin 2
Synonyms: Math4A, ngn2, Atoh4, bHLHa8
Type: Gene
Species: Mouse
Chromosome: 3
Alteration at locus: Conditional
NCBI: 11924
Homologene: 7633
Genetic Alterations
Floxed allele of Neurog2
Genotype Determination
  • Genotyping Protocol
  • Center protocol and contact for technical support will be shipped with mice.
ES Cell Line
R1
Phenotype
None/Normal/Wild-type
Conditional: In retina, fewer rod photoreceptors and excess biopolar neurons. In brain, migration defects in cortical neurons.
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

MeSH Terms
  • Age Factors
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors/metabolism
  • Blotting, Western/methods
  • Cell Count/methods
  • Cell Movement/physiology
  • Cells, Cultured
  • Chickens
  • Cloning, Molecular/methods
  • Dendrites/physiology
  • Electrophoresis, Gel, Pulsed-Field/methods
  • Electroporation/methods
  • Embryo, Mammalian
  • Embryo, Nonmammalian
  • Female
  • Fluorescent Antibody Technique/methods
  • Gene Expression Regulation, Developmental/physiology
  • Green Fluorescent Proteins/metabolism
  • Humans
  • In Vitro Techniques
  • Male
  • Mice
  • Microscopy, Confocal/methods
  • Microtubule-Associated Proteins/metabolism
  • Models, Biological
  • Neocortex/cytology
  • Neocortex/embryology
  • Neocortex/metabolism
  • Nerve Tissue Proteins/metabolism
  • Phosphorylation
  • Pregnancy
  • Pyramidal Cells/cytology
  • Pyramidal Cells/physiology
  • Sequence Alignment
  • Stem Cells/physiology
  • Time Factors
  • Tubulin/metabolism
  • Tyrosine/metabolism
  • rhoA GTP-Binding Protein/metabolism
Strain Development
An FRT-neo-FRT-loxP cassette was inserted in an intron 180 bp 5' to the ATG of the Neurog2 gene. Five myc tags were inserted at the N-terminal end of the coding sequence and fused to the full-length mouse coding sequence (minus the first codon). A loxP site was located 1.3 kb after the stop codon. ES cells were injected into blastocysts from 129X1/SvJ x 129S1/Sv)F1-Kitl+ mating. After germline transmission, mice were bred to CD1 for an unknown number of generations, then intercrossed to create Neurog2flox/flox stock in the lab of N. Brown (UC Davis) until donation to the MMRRC in 2018.
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Developmental Biology
  • Neurobiology
  • Sensorineural
Donor
Francois Guillemot, Ph.D The Francis Crick Institute
Primary Reference

Hand R, Bortone D, Mattar P, Nguyen L, Heng JI, Guerrier S, Boutt E, Peters E, Barnes AP, Parras C, Schuurmans C, Guillemot F, Polleux F. Phosphorylation of Neurogenin2 specifies the migration properties and the dendritic morphology of pyramidal neurons in the neocortex. Neuron. 2005 Oct 6;48(1):45-62. (Medline PMID: 16202708)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
The MMRRC at UC Davis performed microbita analysis on feces samples received from the donating investigator while the mice were still at their facility. There is a MMRRC Fecal Microbiota report available for this strain.If you are interested in having the MMRRC at UC Davis perform microbiota analysis once the line is established at your facility please contact mmrrc@ucdavis.edu.
Coat Color
White
Eye
Pink
MMRRC Breeding System
Sib-mating
Generation
Unknown
Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 6 to 8 months 8 to 12 months
Average litter size
10-14
Recommended wean age
3 Weeks
Average Pups Weaned
10-14

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
050509-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
050509-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.