Strain Name:
STOCK Atm1Brd Opn4tm1c(KOMP)Wtsi/YauMmucd
Stock Number:
050515-UCD
Citation ID:
RRID:MMRRC_050515-UCD
Other Names:
OPN4 floxed

Strain Information

Opn4tm1c(KOMP)Wtsi
Name: opsin 4 (melanopsin); targeted mutation 1c, Wellcome Trust Sanger Institute
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Knockout
Opn4
Name: opsin 4 (melanopsin)
Synonyms: 1110007J02Rik
Type: Gene
Species: Mouse
Chromosome: 14
Alteration at locus: Knockout
NCBI: 30044
VEGA: 14
Homologene: 69152
Atm1Brd
Name: nonagouti; targeted mutation 1, Allan Bradley
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Targeted Mutation
Genetic Alterations
The second exon of the Opn4 gene is flanked by two loxP sites.
Genotype Determination
ES Cell Line
JM8A3.N1 derived from C57BL/6N-Atm1Brd
Mammalian Phenotype Terms
Allelic Composition: Atm1Brd/Atm1Brd (Genetic Background: C57BL/6N-Atm1Brd )

Allelic Composition: Atm1Brd/a (Genetic Background: C57BL/6N-Atm1Brd )

MeSH Terms
  • Acetylcholine/metabolism
  • Animals
  • Atropine/pharmacology
  • Iris/drug effects
  • Light
  • Mice
  • Mice, Transgenic
  • Muscarinic Antagonists/pharmacology
  • Photic Stimulation
  • Signal Transduction/drug effects
Strain Development
Targeted C57BL/6N embryonic stem cells were obtained from UCDAVIS KOMP repository (clone EPD0642_3_D12), and subsequently injected into the B6(Cg)-Tyrc-2J/J blastocysts at the Transgenic Core Facility of Johns Hopkins University School of Medicine. The mice generated from successful germline transmission were heterozygous for the “knock-out first” allele (i.e., Opn4KOF/+). Exposure of the construct to flippase [by breeding with B6.Cg-Tg(ACTFLPe)9205Dym/J (JAX Strain #005703)] resulted in the removal of the trapping cassette and expression of the Opn4 gene. The second exon of Opn4 remained floxed.
Suggested Control Mice
C57BL/6N
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cell Biology
  • Neurobiology
Donor
King Wai Yau, Ph.D., Johns Hopkins School of Medicine.
Qian Wang, Ph.D., Stanford University.
Primary Reference

Wang Q, Yue WWS, Jiang Z, Xue T, Kang SH, Bergles DE, Mikoshiba K, Offermanns S, Yau KW. Synergistic Signaling by Light and Acetylcholine in Mouse IrisSphincter Muscle. Curr Biol. 2017 Jun 19;27(12):1791-1800.e5. doi:10.1016/j.cub.2017.05.022. Epub 2017 Jun 1. (Medline PMID: 28578927)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
Eye
Black
MMRRC Breeding System
Sib-mating
Generation
Not specified. However, the strain is considered B6.Cg because the embryonic cells were of B6N origin and the FLP modifier line was B6.Cg, making it B6-congenic even in absence of backcrossing.
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 6 to 8 months 6 to 8 months
Average litter size
7-9
Recommended wean age
3 Weeks
Average Pups Weaned
5-9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
050515-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
050515-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.