Strain Name:
B6.129X1-Ephx1tm1Gonz/Mmucd
Stock Number:
050526-UCD
Citation ID:
RRID:MMRRC_050526-UCD
Other Names:
Microsomal epoxide hydrolase knockout

Strain Information

Ephx1tm1Gonz
Name: epoxide hydrolase 1, microsomal; targeted mutation 1, Frank J Gonzalez
Synonyms: mEH-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Knockout
Ephx1
Name: epoxide hydrolase 1, microsomal
Synonyms: Eph1, Eph-1, mEH
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Knockout
NCBI: 13849
HGNC: HGNC:3401
Homologene: 94
Genetic Alterations
mEH global knockout. A neomycin resistance cassette replaced 0.7 kb of sequence containing part of exon 2.
ES Cell Line
Unspecified, originating from 129X1/SvJ.
Phenotype
None/Normal/Wild-type
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

  • neoplasm
    • decreased incidence of tumors by chemical induction [] [PMID:10446164]
  • homeostasis/metabolism
    • decreased incidence of tumors by chemical induction [] [PMID:10446164]
MeSH Terms
  • 9,10-Dimethyl-1,2-benzanthracene/metabolism
  • 9,10-Dimethyl-1,2-benzanthracene/toxicity
  • Animals
  • Aryl Hydrocarbon Hydroxylases
  • Carcinogens/toxicity
  • Cytochrome P-450 CYP1B1
  • Cytochrome P-450 Enzyme System/physiology
  • Epoxide Hydrolases/genetics
  • Epoxide Hydrolases/physiology
  • Female
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Microsomes, Liver/enzymology
  • Skin Neoplasms/chemically induced
  • Adult
  • Biopsy, Needle/adverse effects
  • Hemorrhage/etiology
  • Humans
  • Kidney Failure, Chronic/complications
  • Lung Injury
  • Male
Strain Development
ES cell clones (unspecified, of 129X1 origin) heterozygous for the disrupted mEH gene were injected into C57BL/6 blastocysts and transferred into the uterus of pseudopregnant recipient NIH Swiss females to generate chimeric founder mice. Male chimeras were bred with C57BL/6 females. Subsequent backcross to B6 (sub-strain uncertain) for an unknown number of generations until cryo-preservation at NCI. Upon cryo-recovery at NIEHS, the line was backcrossed for 3 generations to C57BL/6NCrl commercial wild-type mice prior to cryo-preservation at the MMRRC.
Suggested Control Mice
Wild-type litter mates from het x het crosses, or commercial C57BL/6NCrl
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cancer
  • Cardiovascular
  • Cell Biology
  • Diabetes
  • Immunology and Inflammation
  • Metabolism
  • Models for Human Disease
  • Research Tools
Donor
Frank J. Gonzalez, Ph.D., National Cancer Institute, National Institutes of Health.
Darryl Zeldin, M.D., NIH.
Primary Reference

Sherman S, Nishio JN, Ravikrishnan KP. Massive pulmonary hemorrhagecomplicating thoracocentesis in chronic renal faiure. Chest. 1979 Jul;76(1):118. (Medline PMID: 0446164)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
Eye
Black
MMRRC Breeding System
Sib-mating
Generation
N5+ (C57BL/6)
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: N/A N/A
Age Reproductive Decline: Undetermined Undetermined
Average litter size
7-9
Recommended wean age
3 Weeks
Average Pups Weaned
5-9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
050526-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.