Strain Detail Sheet

Strain Name:
B6J.Cg-Nkx2-9tm1Jlck/Mmmh
Stock Number:
050535-MU
Citation ID:
RRID:MMRRC_050535-MU
Other Names:
Nkx2.9-/-

Strain Information

Gene Details

Nkx2-9tm1Jlck
Name: NK2 homeobox 9; targeted mutation 1, Joseph Locker
Synonyms: Nkx2.8-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Knockout
Nkx2-9
Name: NK2 homeobox 9
Synonyms: tinman, Nkx-2.9, Nkx2.9
Type: Gene
Species: Mouse
Chromosome: 12
Alteration at locus: Knockout
NCBI: 18094
Homologene: 7444
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Genetic Alterations
The coding region of the first exon of the Nkx2-9 gene was replaced by a nuclear-localizing LacZ gene, placed under control of the natural promoter and translation start.
Genotype Determination
ES Cell Line
WW6

Strain Description

Phenotype
Mice have a null allele of Nkx2-9, made by replacement of the gene with a promoter controlled-LacZ marker gene, which marks cell populations in the lungs, thyroid, brain and other tissues. In the lung, the marked cells appear to be stem/progenitor cells, primarily in large airways. Development is normal, with some changes in brain architecture, where the marker is found in the ventral pole of the neural tube, as ependymal cells, small periependymal cells, and related neurons in the mature brain. Compared to the heterozygotes, the homozygous knockouts have an increased number of neurons in extending from the periventricular regions of the lateral ventricles, the hypothalamus, midbrain, and spinal cord. In the lungs, small LacZ-marked cells accumulate in the bronchi, progressing to bronchial dysplasia in adult mice, and cancer in aged mice. In the thyroid, the marker localizes to a small number of follicular and parafollicular cells.
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )
MeSH Terms
  • Alleles
  • Animals
  • Bronchi/pathology
  • Cell Proliferation
  • DNA-Binding Proteins/analysis
  • Female
  • Homeodomain Proteins/analysis
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/physiology
  • Liver Neoplasms, Experimental/etiology
  • Lung/embryology
  • Lung/pathology
  • Lung Neoplasms/etiology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Stem Cells/cytology
  • Transcription Factors/analysis
  • Transcription Factors/genetics
  • Transcription Factors/physiology
Strain Development
Knockout in WW6 embryonic stem cells via homologous recombination. Injection in C57BL/6 blastocysts. Implanted into 129SvJ females. Chimeras were then bred to homozygosity in C57BL/6J (JAX Strain #000664) and further bred >10 generations in that strain. Maintained as homozygous knockout strains; breeding with purchased wild-type to generate heterozygotes. Also available on A/J (N9+) from the same donor.
Suggested Control Mice
Wild-type

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Research Applications

  • Cancer
  • Developmental Biology
  • Internal/Organ
  • Models for Human Disease
  • Neurobiology

Strain Origin

Donor
Joseph Locker, M.D., University of Pittsburgh.
Primary Reference

Tian J, Mahmood R, Hnasko R, Locker J. Loss of Nkx2.8 deregulates progenitorcells in the large airways and leads to dysplasia. Cancer Res. 2006 Nov1;66(21):10399-407. (Medline PMID: 17079460)

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black
Eye
Black
Other
In addition to our lung/cancer studies, the mouse has been used for studies of motor axon exit from the developing spinal cord (Bravo-Ambrosio A et al., Development 139:1345, 2012) and an active study of midbrain development in collaboration with Willemieke Kouwenhoven and Marten Smidt, University of Amsterdam. The mutation has been fully backcrossed into B6 and also into cancer-sensitive A/J.

Breeding

MMRRC Breeding System
Random intra-strain mating
Generation
N10+ (C57BL/6J)
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 6 to 8 months 6 to 8 months
Bred to Homozygosity
Yes
Average litter size
7-9
Recommended wean age
3 Weeks
Average Pups Weaned
5-9

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.