Strain Name:
C57BL/6N-Gt(ROSA)26Sortm1(CAG-IL37)Osjyz/Mmnc
Stock Number:
050607-UNC
Citation ID:
RRID:MMRRC_050607-UNC
Other Names:
tg IL-37b WT

Strain Information

Gt(ROSA)26Sortm1(CAG-IL37)Osjyz
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1; targeted mutation 1, Yizu Jiao and Steve Offenbacher
Type: Allele
Species: Multi-species
Chromosome: 6
CAG
Name: CMV early enhancer/chicken beta actin promoter/rabbit beta globin splice acceptor
Type: DNA Segment
Species: Multi-species
Chromosome:
Gt(ROSA)26Sor
Name: gene trap ROSA 26, Philippe Soriano
Synonyms: ROSA26, beta geo, Gtrgeo26, Gtrosa26, R26, Thumpd3as1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14910
IL37
Name: interleukin 37
Type: Gene
Species: Homo sapiens (human)
Chromosome: 2
Genetic Alterations
Targeted transgenic expression of human IL37, driven by the CAG promoter, in the Rosa26 locus.

IL37 is located within the IL-1 gene cluster on 2q12, is a regulatory cytokine that dampens the immune response in humans. These loci have also been associated with several inflammatory pathogenetic conditions including Alzheimer’s disease, End-stage Renal Disease (ESRD)12, osteoarthritis16, coronary artery disease17, type I diabetes mellitus, stroke, and periodontal disease. However, mice do not have this gene and its homologs. This and related strains (MMRRC:50610, 50611, 50612) are available to study the role of IL37 in periodontal disease and possibly other inflammatory and autoimmune disorders.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    C57BL/6N-PRX-B6N #1
    Phenotype
    Strong constitutive production of human IL-37 protein in unstimulated blood samples.
    Strain Development
    Wild-type (or variant) human IL37 cDNA was cloned into a ROSA26-CAGG targeting vector. Constructs were validated by sequencing to confirm the insertion of the correct sequence. Each construct was electroporated into C57BL/6N-PRX-B6N #1 mES cells (Primogenix) and homologous integrant clones were validated by Southern blot analysis. Correctly targeted clones were microinjected into C57BL/6-albino blastocysts for chimera production. Chimeras were mated to C57BL/6N females and germline transmission was confirmed by PCR genotyping.

    Note: This strain belongs to a group of related IL37 models: MMRRC:50607, 50610, 50611, and 50612.
    Suggested Control Mice
    C57BL/6N
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Immunology and Inflammation
    • Models for Human Disease
    Donor
    Yizu Jiao, M.D., Univeristy of North Carolina School of Dentistry.
    Steven Offenbacher, M.D., The University of North Carolina Chapel Hill.
    Primary Reference

    Offenbacher S, Jiao Y, Kim SJ, Marchesan J, Moss KL, Jing L, Divaris K,Bencharit S, Agler CS, Morelli T, Zhang S, Sun L, Seaman WT, Cowley D, Barros SP,Beck JD, Munz M, Schaefer AS, North KE. GWAS for Interleukin-1β levels ingingival crevicular fluid identifies IL37 variants in periodontal inflammation.Nat Commun. 2018 Sep 11;9(1):3686. doi: 10.1038/s41467-018-05940-9. (Medline PMID: 30206230)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
    Coat Color
    Black
    Eye
    Black
    MMRRC Breeding System
    Sib-mating
    Generation
    N/A
    Overall Breeding Performance
    Excellent
    NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Hetero/Hemizygotes are fertile: Yes Yes
    Age Reproductive Decline: 6 to 8 months 6 to 8 months
    Average litter size
    7-9
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    5-9

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    050607-UNC-EMBRYO Cryo-preserved embryos $1,404.00 / $2,331.00
    Non-Profit / For-Profit
    Aliquot Approximate quantity2 : 20-40 embryos / aliquot
    050607-UNC-SPERM Cryo-preserved spermatozoa $564.00 / $937.00
    Non-Profit / For-Profit
    Aliquot Approximate quantity3
    050607-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / $4,837.00
    Non-Profit / For-Profit
    Litter Recovered litter4; additional fees for any special requests.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.