Strain Detail Sheet

Strain Name:
B6J.129S4(C)-Myo1ctm1.1Pgg/Mmucd
Stock Number:
050614-UCD
Citation ID:
RRID:MMRRC_050614-UCD
Other Names:
Y61G Myo1c

Strain Information

Gene Details

Myo1ctm1.1Pgg
Name: myosin IC; targeted mutation 1.1, Peter G Gillespie
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Knock-In
Myo1c
Name: myosin IC
Synonyms: myosin-Ibeta, myr2, C80397, mm1beta
Type: Gene
Species: Mouse
Chromosome: 11
Alteration at locus: Knock-In
NCBI: 17913
HGNC: HGNC:7597
Homologene: 32046
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Genetic Alterations

Targeted knock-in of a Y61G substitution in both copies of the Myo1c gene.

HGVS nomenclature:
  • Genbank RefSeq - mRNA: NM_001080774.1 (variant 2, **not** the longest isoform)
  • Genbank RefSeq, protein: NP_001074243.1
  • Variant, nucleic acid level: c.181_183delTACinsGGA
  • Variant, amino acid level, predicted: p.Tyr61Gly
    Check this variant: LUMC Mutalyzer
Note: When this strategy was devised, there was only one known isoform of myosin 1c, which was then known as myosin Iβ (PMID:10531338). Subsequently, the protein was renamed myosin 1c, but there still was only one known isoform (PMID:11853671 and PMID:16102537). The second isoform was identified later. However, by being longer, the longer isoform is referred to as isoform a (NM_001080775.1), and the one that this model's numbering is based on is isoform c (NM_001080774.1).
ES Cell Line
Not specified

Strain Description

Phenotype
mutation sensitizes Myo1c to N6-modified ADP analogs
MeSH Terms
  • Adaptation, Physiological/physiology
  • Alleles
  • Animals
  • Anura
  • Hair Cells, Vestibular/metabolism
  • Mechanotransduction, Cellular/physiology
  • Membrane Potentials/drug effects
  • Membrane Potentials/genetics
  • Mice
  • Mice, Transgenic
  • Mutation/genetics
  • Myosin Type I
  • Myosins/genetics
  • Myosins/metabolism
  • Patch-Clamp Techniques
  • Postural Balance/physiology
  • Protein Synthesis Inhibitors/pharmacology
  • Reaction Time/physiology
  • Time Factors
Strain Development
(Adopted from PMID:16102537): We generated the targeting construct using a 6.5 kbp NotI–PstI fragment of the BamHI genomic clone described previously (PMID:11853671). Tyrosine 61 was converted to glycine by changing the codon to GGA; to permit efficient screening, we added additional silent mutations ~30 bp from the Y61G mutation. The neomycin-resistance positive selection cassette, flanked with loxP sites, was inserted into a NheI site located in the large intron between exons 4 and 5 (see diagram in Holt et al., 2002). The short homology arm was thus ~0.5 kb and the long arm ~3 kb. We also added a negative selection cassette containing the diphtheria toxin gene, outside the region of homology. ES cells (129S4 clone) were electroporated with the targeting construct, and two positive clones were identified by PCR and Southern blot screening. Clones were injected into blastocysts, and one chimeric mouse was identified that transmitted the mutation to subsequent generations. Animals heterozygous for the floxed allele were bred to a Cre deleter mouse (JAX Strain #006054), which expresses Cre recombinase ubiquitously from the two-cell stage using the human cytomegalovirus promoter. Experiments described here used homozygous Y61G mice with or without the loxP-Neo cassette present. Although Neo can act in cis to suppress the expression of nearby genes, we found no evidence of that effect in our experiments. The Y61G mice were backcrossed to C57BL/6J (>10 generations). Although C57BL/6 mice suffer age-related hearing loss due to the ahl1 mutation of cadherin 23, our experiments exclusively used early postnatal mouse pups with no hearing loss. We confirmed that Y61G and C57BL/6 control mice had normal auditory brainstem responses (ABRs) at >21 days.
Suggested Control Mice
C57BL/6J

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Research Applications

  • Developmental Biology
  • Internal/Organ
  • Neurobiology
  • Sensorineural

Strain Origin

Donor
Peter Barr-Gillespie, Ph.D., Oregon Health & Science University.
Primary Reference

Stauffer EA, Scarborough JD, Hirono M, Miller ED, Shah K, Mercer JA, Holt JR, Gillespie PG. Fast adaptation in vestibular hair cells requires myosin-1cactivity. Neuron. 2005 Aug 18;47(4):541-53. (Medline PMID: 16102537)

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Backcross
Generation
N12 (C57BL/6J)
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 6 to 8 months 8 to 12 months
Bred to Homozygosity
Yes
Average litter size
7-9
Recommended wean age
3 Weeks
Average Pups Weaned
5-9

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
050614-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $869.88
Non-Profit / For-Profit		 Aliquot Approximate quantity3
050614-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit		 Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.