Strain Name: B6.Cg-Tg(CAG-OTC/CAT)4033Prab/Mmmh
Stock Number: 011195-MU
Other Names:
Gene Details: (provided by MGI)
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Transgene: Tg(CAG-OTC/CAT)4033Prab Name: transgene insertion 4033, Peter S Rabinovitch Alteration at locus: Transgenic |
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Transgene: CAT (Human) Name: catalase Alteration at locus: Transgenic |
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Promoter: CAG (Chicken/Rabbit) Name: CMV early enhancer/chicken beta actin promoter/rabbit beta globin splice acceptor Alteration at locus: Transgenic |
Genetic Alterations:
To generate the MCAT transgene, the 11 carboxy-terminal amino acids, including the peroxisomal
localization signal, were deleted from the human catalase gene. The initiating methionine was
also deleted and the first 25 amino acids of the ornithine transcarbamylase leader sequence were
added to the amino terminus to target this protein product to mitochondria. The catalase cDNA
construct was then cloned into a plasmid under the influence of the chicken-actin promoter/CMV
enhancer (CAG) and the construct was introduced into B6C3H X B6 hybrid embryos.
Genotype Determination:
ES Cell Line: Not Applicable
Strain Description
Phenotype
Homozygous phenotype: Unknown
Hemizygous phenotype: Transgenic mice with targeted overexpression of catalase in mitochondria show lifespan extension,
reduced aging pathology and reduced susceptibility to oxidative damage. Median and maximum life
spans were maximally increased (average 5 months, and 5.5 months, respectively) in MCAT animals.
Cardiac pathology and cataract development were delayed, oxidative damage (8-hydroxyguanosine)
was reduced, H2O2 production and H2O2-induced aconitase inactivation were attenuated, and the
development of mitochondrial deletions measured by LX-PCR was reduced.
Founder genetic background: (C57BL/6 x C3H)F1
Strain genetic background: C57BL/6
Strain Development: G1 - G12 mCAT Tg male x Bl/6 female G13 mCAT Tg male x mCAT Tg female to check congenity on Bl/6 background G14 mCAT Tg male x Bl/6 female
Suggested Control Mice:
- Wildtype littermates
Research Applications
- Cardiovascular
- Diabetes/Obesity
- Neurobiology
- Oxidative Stress
Strain Origin
Donor: Warren C. Ladiges, DVM, University of Washington
Primary Reference:
Schriner SE, Linford NJ, Martin GM, Treuting P, Ogburn CE, Emond M, Coskun PE, Ladiges W, Wolf N, Van Remmen H, Wallace DC, Rabinovitch PS. Extension of murine life span by overexpression of catalase targeted to mitochondria. Science. 2005 Jun 24;308(5730):1909-11. Epub 2005 May 5. (Medline PMID: 15879174)
Special Considerations
None
Health Status Report
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Order Request Information
Availability Level:
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Conditions of Distribution:
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Fees:
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
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Distribution Fee/unit (US $) |
Units | Notes | ||||
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| Litter | Recovered litter1; additional fees for any special requests. | ||||
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| Aliquot | Approximate quantity.2 | ||||
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| Aliquot | Approximate quantity3: 20-40 embryos / aliquot | ||||
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.
The MMRRC is a collaborative effort, funded by grants from the NIH
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