Strain Name: C57BL/6-Mitfmi-ew/Mitfmi-sp/Mmmh
Stock Number: 000164-MU
Other Names:
Gene Details: (provided by MGI)
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Allele Symbol: Mitfmi-ew Name: eyeless white Chromosome: 6 Alteration at locus: Spontaneous Mutation |
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Allele Symbol: Mitfmi-sp Name: microphthalmia spotted Chromosome: 6 Alteration at locus: Spontaneous Mutation |
Genetic Alterations:
A to T transversion four nucleotides downstream of the exon 6b
donor site. This mutation is likely to be sufficient to abolish
normal splicing, leading to exon skipping and an mRNA missing
exons 6a/6b.
Genotype Determination:
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- view also: Heterozygote and Wildtype
- view also: Heterozygotes
ES Cell Line: Not applicable
Strain Description
Phenotype
Description of White Spotting mutants
Homozygous mice are white and have severe micropthalmia such that
the eyes are not grossly visible (see also MMRRC:000162). Heterozygous mice are
predominantly white with a few black spots.
Founder genetic background: C57BL/6Bn
Strain genetic background: C57BL/6-Mitfmi-sp (MMRRC:000153)
Strain Development: Spontaneous mutation arose on the C57BL/6Bn strain and was subsequently backcrossed onto the C57BL/6-Mitfmi-sp. Heterozygotes are sib mated to produce homozygotes when needed.
Suggested Control Mice:
- Wildtype littermates
- MMRRC:000153
Research Applications
- Melanocyte development and survival
- Eye development
Strain Origin
Donor: Dr. M. L. Lamoreux, Texas A&M University
Primary Reference:
- Hallsson JH, Favor J, Hodgkinson C, Glaser T, Lamoreux ML, Magnusdottir R, Gunnarsson GJ, Sweet HO, Copeland NG, Jenkins NA, Steingrimsson E. Genomic, transcriptional and mutational analysis of the mouse microphthalmia locus. Genetics. 2000 May;155(1):291-300. (Medline: 10790403)
- Steingrimsson E, Arnheiter H, Hallsson JH, Lamoreux ML, Copeland NG, Jenkins NA. Interallelic complementation at the mouse mitf locus. Genetics. 2003 Jan;163(1):267-76. (Medline: 12586714)
Colony and Husbandry Information
Appearance
Coat color: White
Other:
Breeding
MMRRC Breeding System: Backcross
Breeding Scheme(s):
- Heterozygous Female x C57BL/6-Mitfmi-sp Male
- C57BL/6-Mitfmi-sp Female x Heterozygous Male
- Heterozygous Female x Heterozygous Male (to obtain Homozygous mice)
Generation: N10+
Overall Breeding Performance: Good
Reproductive Statistics
| Viability and Fertility: | Female | Male |
|---|---|---|
| Homozygotes are viable: | Yes | Yes |
| Homozygotes are fertile: | Poor | Poor |
| Heterozygotes are fertile: | Yes | Yes |
| Age Reproductive Decline: | Unknown | Unknown |
Average litter size: 7
Recommended wean age: 4 weeks
Special Considerations
Feed mouse chow that contains 9 percent fat for breeding animals.
Health Status Report
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Order Request Information
Availability Level:
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Conditions of Distribution:
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Fees:
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
| Click button to Request this one strain.
(Use the MMRRC Catalog Search to request more than one strain.) | |||||||
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Distribution Fee/unit (US $) |
Units | Notes | ||||
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| Litter | Recovered litter1; additional fees for any special requests. | ||||
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| Aliquot | Approximate quantity.2 | ||||
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| Aliquot | Approximate quantity3: 20-40 embryos / aliquot | ||||
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.
The MMRRC is a collaborative effort, funded by grants from the NIH
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