Allele Symbol: Mbd1tm1Fhg
Name: targeted mutation 1, Fred H Gage
Alteration at locus: Knockout
Generation of MBD1
ES Cell Line: J1 derived from C57BL/6
Homozygous phenotype: Histological analysis indicated that MBD1-/- mice had no detectable developmental defects. Therefore, donor focused analysis on adult mice. Adult MBD1-/- mice appear healthy and fertile, with a normal life span. Donor performed a battery of neurological tests and found no significant defects in adult MBD1-/- mice. However, the animal showed deficits in adult neurogenesis and hippocampal function (described in Zhao, X et al PNAS 2003).
Heterozygous phenotype: Not applicable
Founder genetic background: 129
Strain genetic background: C57BL/6
Strain Development: Females MBD1+/- were crossed to male C57BL/6
Suggested Control Mice:
Donor: Fred H Gage, Ph.D., The Salk Institute
Zhao X, Ueba T, Christie BR, Barkho B, McConnell MJ, Nakashima K, Lein ES, Eadie BD, Willhoite AR, Muotri AR, Summers RG, Chun J, Lee KF, Gage FH. Mice lacking methyl-CpG binding protein 1 have deficits in adult neurogenesis and hippocampal function. Proc Natl Acad Sci U S A. 2003 May 27;100(11):6777-82. Epub 2003 May 14. (Medline PMID: 12748381)
According to donor, female +/- with male +/- gives the best results
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email email@example.com.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
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|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
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