Strain Name: B6.Cg-Tg(tetO-Mrgpra1)1Kdmc/Mmmh
Stock Number: 029882-MU
Other Names:
Gene Details: (provided by MGI)
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Transgene: Tg(tetO-Mrgpra1)1Kdmc Name: transgene insertion 1, Ken D McCarthy Alteration at locus: Transgenic |
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Promoter: tetO (Synthetic) Name: tetracycline operon Alteration at locus: Transgenic |
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Transgene: Mrgpra1 Name: MAS-related GPR, member A1 Chromosome: 7 Alteration at locus: Transgenic |
Genetic Alterations:
Transgenic line
carrying tetO promoter driven MrgA1 gene. MrgA1 is a Gq GPCR. DNA
construct was microinjected into fertilized oocytes (C3H/C57).
Genotype Determination:
ES Cell Line: Not applicable
Strain Description
Phenotype
Homozygous phenotype: none
Hemizygous phenotype: none
Founder genetic background: C57BL6/C3H
Strain genetic background: C57BL/6J
Strain Development: Founder animal was bred to tTA containing line to analyze expression. Offspring were interbred to perform experiments. Subsequent offspring were crossed with C57BL/6J for backcrossing.
Suggested Control Mice:
- Wildtype littermates
Research Applications
- Cell Biology
- Neurobiology
- Research Tools
Strain Origin
Donor: Ken D McCarthy, Ph.D., University of North Carolina, Chapel Hill
Primary Reference:
Fiacco TA, Agulhon C, Taves SR, Petravicz J, Casper KB, Dong X, Chen J, McCarthy KD. Selective stimulation of astrocyte calcium in situ does not affect neuronal excitatory synaptic activity.Neuron. 2007 May 24;54(4):611-26. (Medline PMID: 17521573)
Special Considerations
None
Health Status Report
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Order Request Information
Availability Level:
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Conditions of Distribution:
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Fees:
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
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Distribution Fee/unit (US $) |
Units | Notes | ||||
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| Litter | Recovered litter1; additional fees for any special requests. | ||||
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| Aliquot | Approximate quantity.2 | ||||
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.
The MMRRC is a collaborative effort, funded by grants from the NIH
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