Strain Detail Sheet

Strain Name: FVB/N-Tg(tetO-HTR4*D100A)7Niss/Mmmh

Stock Number: 029992-MU

Other Names:

Gene Details: (provided by MGI)

Transgene: Tg(tetO-HTR4*D100A)7Niss
Name: transgene insertion 7, Robert A Nissenson
Alteration at locus: Transgenic

Promoter: tetO (Synthetic)
Name: tetracycline operon
Alteration at locus: Transgenic

Transgene: HTR4*D100A (Human)
Name: 5-hydroxytryptamine (serotonin) receptor 4 - D100A
Chromosome: 5
Alteration at locus: Transgenic

Genetic Alterations:
This is a transgenic mouse line carrying the TetO-Rs1 transgene. Rs1 is an engineered G-protein coupled receptor based on the human 5HT4b serotonin receptor but with a D100A mutation that renders it insensitive to serotonin. Rs1 is linked to the TetO tetracycline transactivator promoter. Tetracycline-dependent regulation of Rs1 expression is achieved when this TetO-Rs1 mouse line is mated to a second transgenic line carrying a tTA or rtTA transactivator protein.

Genotype Determination:

Donor's PCR

ES Cell Line: Not applicable

Strain Description

Phenotype
Homozygous phenotype: Does not appear to have a gross phenotype. Does appear to have normal fertility and lifespan.

Hemizygous phenotype: No identified phenotype as a single transgenic.

Founder genetic background: FVB/N

Strain genetic background: FVB/N

Strain Development: This transgenic mouse line was generated by pronuclear injection of a construct carrying the TetO-Rs1 transgenic construct. Mice have been maintained on a FVBN background.

Suggested Control Mice:

Wildtype littermates

Research Applications

Cardiovascular
Cell Biology
Developmental Biology
Endocrine Deficiency
Models for Human Disease
Neurobiology
Research Tools

Strain Origin

Donor: Bruce Conklin, Ph.D., Gladstone Institute of Cardiovascular Disease

Primary Reference:

Hsiao EC; Boudignon BM; Chang WC; Bencsik M; Peng J; Nguyen TD; Manalac C; Halloran BP; Conklin BR; Nissenson RA, Osteoblast expression of an engineered Gs-coupled receptor dramatically increases bone mass., Proc Natl Acad Sci U S A 2008 Jan 29;105(4):1209-14 (Medline PMID: 18212126)

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Availability Level:

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution:

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees:

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)

MMRRC Item #	Description

Distribution
Fee/unit (US $)

Units

Notes

029992-MU-RESUS

Litter recovered from cryo-archive

$2,022.00 / $4,109.00

Non-Profit / For-Profit

Litter

Recovered litter¹; additional fees for any special requests.

029992-MU-SPERM

Cryo-preserved spermatozoa

$437.00 / $817.00

Non-Profit / For-Profit

Aliquot

Approximate quantity.²

¹ The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

² An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

³ An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the "Request this Strain" button above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.