Allele Symbol: Bgntm1Mfy
Name: targeted mutation 1, Marian F Young
Alteration at locus: Knockout
neo casette insertion into exon 2 just 5' to the start of translation
ES Cell Line: J1 derived from C57BL/6
Homozygous phenotype: Age dependent osteoporosis starting in homozygous females after 3 months of age (judged by X-ray and histomorphometry). Mild muscular dystrophy like sydrome (judged by ultrastructure of tissue) thinned skin in phenotype like mild osteogenesis imperfecta (judged by physical weakness and histology).
Hemizygous phenotype: Age dependent osteoporosis starting in hemizygous males after 3 months of age (judged by X-ray and histomorphometry). Mild muscular dystrophy like sydrome (judged by ultrastructure of tissue) thinned skin in phenotype like mild osteogenesis imperfecta (judged by physical weakness and histology).
Founder genetic background: C3H
Strain genetic background: C57BL/6
Strain Development: Donor received C3.129S4(B6)-Bgn/Mmmh mice from the MMRRC (stock number 000040-MU) several years ago and bred them onto the C57BL/6 background.
Suggested Control Mice:
Donor: Sean Morrison, Ph.D., University of Michigan
Kiel MJ, Radice GL, Morrison SJ. Lack of evidence that hematopoietic stem cells depend on N-cadherin-mediated adhesion to osteoblasts for their maintenance. Cell Stem Cell. 2007 Aug 16;1(2):204-17. (Medline PMID: 18371351)
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email email@example.com.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
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|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
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