Name: targeted mutation 2, Guoqiang Gu
Alteration at locus: Knock-In
A tTA-svPolyA cDNA replaced the entire coding region of the Neurog 3 locus.
ES Cell Line: TL-1 derived from mixed
Homozygous phenotype: Mice lack endocrine islet cells. lack some nerve cells in the neural tubes.
Heterozygous phenotype: Mice have glucose tolerance problem; otherwise healthy and fertile.
Founder genetic background: 129S6/SvEvTac
Strain genetic background: mixed
Strain Development: Knockin ES cells were derived from 129S6/svEVTac strains. Chimera mice were derived and crossed with C56BL/6 animals to obtain germ line transmission. Animals were then crossed with CD1 mice for regular maintenance.
Suggested Control Mice:
Donor: Guoqiang Gu, Ph.D., Vanderbilt University Medical Center
Wang S; Jensen JN; Seymour PA; Hsu W; Dor Y; Sander M; Magnuson MA; Serup P; Gu G, Sustained Neurog3 expression in hormone-expressing islet cells is required for endocrine maturation and function., Proc Natl Acad Sci U S A 2009 Jun 16;106(24):9715-20 (Medline PMID: 19487660)
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1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
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