Allele Symbol: Tg(Per1-Venus)33Obr
Name: transgene insertion 33, Karl Obrietan
Alteration at locus: Transgenic
A bacterial articificial chromosone (BAC) #RP24-277K16 containing the mouse Per1 gene was modified by the introduction of a Venus (yellow fluorescent protein)-NLS-PEST fusion gene. To improve the kinetics of YFP, the PEST domain of the mouse ornithine carboxylase gene was added to the penultimate codon of Venus and a nuclear localization sequence (NLS) was cloned in-frame between the Venus coding sequence and the PEST domain. The fusion gene contains an hCMV promoter and a SV40 polyadenylation site.
ES Cell Line: Not Applicable
Homozygous phenotype: Not evaluated.
Hemizygous phenotype: Mice that are hemizygous for the clock fluorescent reporter transgene are viable and fertile, normal in size and do not display any gross physical or behavioral abnormalities. The mPeriod1-Venus transgene has widespread expression in multiple brain regions (high expression in: suprachiasmatic nuclei (SCN), Purkinje cells, cerebellum, cerebral cortex and hippocampus) and parallels Per1 expression. In addition, the transgene recapitulates Per1 rhythmic and light-inducible expression in the SCN. This mutant mouse strain may be useful for in vivo imaging to monitor circadian clock timing.
Founder genetic background: FVB/N
Strain genetic background: B6.FVB
Strain Development: The BAC was microinjected into the pronuclei of oocytes from FVB/N females. Founder line 33 was established and bred to C57BL/6J mice to generate the colony. Mice were subsequently backcrossed to C57BL/6J mice for three generations. mice were bred to C57BL/6J for at least 1 generation to establish the colony.
Suggested Control Mice: Wild-type littermates
Donor: Karl Obrietan, Ph.D., Ohio State University
While maintaining a live colony, these mice are bred as hemizygotes.
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email email@example.com.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
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Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
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