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• BayGenomics ES cell lines

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• UNC Mutagenized ES cell lines

• ES Cell Line Information

 

MMRRC / Sanger Institute Gene Trap Resource ES Cell Lines

The Sanger Institute Gene Trap Resource (SIGTR) mouse ES cell line collection was developed to provide a powerful and cost effective approach using gene trapping to create large numbers of insertional mutations that are immediately accessible to molecular characterization. This resource is based on a new generation of gene trap vector designs that are equipped with site-specific recombination sites for post-insertional modification of the trapped locus to create other desired alleles.

Users of SIGTR ES cell lines are advised to follow the protocols available on the SIGTR website. In addition to individual protocols, all the procedures and protocols can be downloaded from the General Information section of the website. Detailed information contained there includes:

  • Molecular confirmation of gene trap insertions
  • Cell culture media and reagent preparation
  • Thawing and growing feeder-independent ES cell lines
  • X-gal staining ES cells
  • Sub-cloning gene trap cell lines

Obtaining SIGTR ES cell lines requires that the requester complete a “Sanger Institute Gene Trap MTA".

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Last Modified: March 16, 2007