MMRRC ES Cell Clones and Related Cell Lines

Through special agreements with originators, the MMRRC distributes mutant ES cell clones, parental and feeder cell lines. Individual facilities may also provide additional services, such as:

• supply chimeric mice created by microinjection;
• breed chimeric mice to establish germline transmission.

Sequencing:

An expansion of an ES cell clone is a minimal service and does not include verification of the identity of the ES cell line by DNA sequencing. As we have occasionally encountered incorrectly identified ES cell clones, this option should be considered only if you are in a hurry and you are confident in your own ability to verify the identity of the ES cell clone. It usually takes between 4-6 weeks to receive the cell line if you choose this option.

We recommend that you have the cell line sequence verified if you will not perform the verification at your facility. No charges will apply if we are unable to confirm the identity of the requested ES cell line. Expansion plus verification of an ES cell line by DNA sequencing requires up to 3 months.

Please see our Fees page.

Three types of parental ES cell lines are available for BayGenomics, Sanger (SIGTR), and Soriano cell line collections:
1) CTRL. These are high passage wild type parental clones suitable for DNA extraction, control purposes, but not for germline transmission.
2) CELL. These are low passage wild type parental clones suitable for a single electroporation and germline transmission.
3) LOW. For both BayGenomics parental ES cell line E14Tg2a.4 (also used for Sanger (SIGTR) lines) and Soriano parental ES cell line AK7, ultra low passage cells suitable for multiple electroporations and germline transmission are available.

Feeder cell lines are also available.

Collection:	Parental line ID:
BayGenomics Sanger (SIGTR) Soriano	MMRRC:015890 MMRRC:015890 (same as BayGenomics) MMRRC:015891

Requesting ES cell lines or chimeric mice made with a cell line, parental lines or a feeder line:

Please use the "Requesting Stocks" navigation tab in the menu bar at the top of this page for information about requesting MMRRC products.